Interactions between lipopolysaccharides and blood factors on the stimulation of equine polymorphonuclear neutrophils
| Autor: | H. Benbarek, Isabelle Caudron, Maurice Lamy, Ginette Deby-Dupont, Sigrid Grulke, Didier Serteyn, Christiane Deby |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Lipopolysaccharides
Lipopolysaccharide Neutrophils Immunology Stimulation Biology Neutrophil Activation chemistry.chemical_compound medicine Animals Horses Whole blood Differential centrifugation chemistry.chemical_classification Reactive oxygen species Dose-Response Relationship Drug General Veterinary Albumin Blood Physiological Phenomena Molecular biology Respiratory burst chemistry Biochemistry lipids (amino acids peptides and proteins) Polymyxin B medicine.drug |
| Zdroj: | Veterinary Immunology and Immunopathology. 64:313-322 |
| ISSN: | 0165-2427 |
| Popis: | In horses, the mechanisms of lipopolysaccharide (LPS) stimulation of isolated neutrophils to produce reactive oxygen species remain unknown. We re-investigated this problem by monitoring the luminol-enhanced chemiluminescence (CL) produced by LPS-stimulated equine neutrophils. The neutrophils were isolated from horse blood by discontinuous density gradient centrifugation (or = 99% neutrophils; viabilityor = 98%). Increasing concentrations of Escherichia coli (E. coli) LPS (from 0.01-10 microg ml(-1)) were used to activate the neutrophils. When LPS was used directly, without another stimulator, the respiratory burst of neutrophils was not activated (N=12 horses; n=5 assays per horse). On the contrary, when LPS was added to whole blood, the neutrophils isolated from this blood were stimulated in a LPS dose-dependent manner, but polymyxin B added to whole blood suppressed this stimulation (N=2; n=6). LPS dissolved in autologous equine plasma stimulated the isolated neutrophils in a dose-dependent manner from 0.1-10 microg ml(-1) (N=5; n=12). Heat inactivation of the plasma abolished this CL increase (N=2; n=5). LPS added to equine albumin did not stimulate the isolated neutrophils (N=2; n=5). On the contrary, the addition of gamma-globulins (1 mg ml(-1)) to LPS (10 microg ml(-1)) led to the stimulation of neutrophils (N=2; n=5). We concluded that LPS did not directly stimulate the isolated equine neutrophils, but that plasmatic factors are needed for the stimulation of these cells by LPS. |
| Databáze: | OpenAIRE |
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