Site-Selectively Coated, Densely-Packed Microprojection Array Patches for Targeted Delivery of Vaccines to Skin
| Autor: | Sally R. Yukiko, Xianfeng Chen, Emily J. Fairmaid, Holly J. Corbett, Anthony P. Raphael, Lorena E. Brown, Tarl W. Prow, Mark A. F. Kendall, Germain J. P. Fernando |
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| Přispěvatelé: | Chen, Xianfeng, Corbett, Holly J, Yukiko, Sally, Raphael, Anthony P, Fairmaid, Emily J, Prow, Tarl W, Brown, Lorena E, Fernando, Germain JP, Kendall, Mark AF |
| Rok vydání: | 2010 |
| Předmět: |
biomedical applications
Materials science Influenza vaccine Chemistry Multidisciplinary Materials Science Materials Science Multidisciplinary Nanotechnology engineering.material Physics Applied Biomaterials Immune system Antigen Coating functional coatings Electrochemistry Nanoscience & Nanotechnology biology Chemistry Physical Physics Limiting vaccine delivery Condensed Matter Physics Electronic Optical and Magnetic Materials Chemistry Ovalbumin Physics Condensed Matter Vaccination Campaigns drug delivery Drug delivery biology.protein Biophysics engineering Science & Technology - Other Topics |
| Zdroj: | Advanced Functional Materials. 21:464-473 |
| ISSN: | 1616-301X |
| DOI: | 10.1002/adfm.201000966 |
| Popis: | Densely packed dry-coated microprojections are shown to deliver vaccines to targeted locations within the skin that are rich in immune cells, thus inducing protective immune responses against a lethal virus challenge. Selectively limiting the antigen coating to the tips of the projections, which penetrate the skin, would significantly reduce the amount of vaccine required in immunization. In this paper a simple technique, dip-coating the microprojections, is introduced to meet this goal. By increasing the coating solution viscosity, an otherwise strong capillary action is mitigated and the desired controlled coating length on projections is achieved. Following application to the skin, most of the coated vaccine material is rapidly released from the projections (82.6% in mass within 2 min) to the target locations within the skin strata and a potent immune response is induced when a conventional influenza vaccine (Fluvax) is tested in a mouse model. The utility of this coating approach to a variety of molecules representative of vaccines (e. g., chicken egg ovalbumin (OVA) protein, DNA, and fluorescent dyes) is demonstrated. These collective attributes, together with the simplicity of the approach, position the dip-coating method for practical utility in large vaccination campaigns. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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