Genistein suppresses the inflammation and GSK-3 pathway in an animal model of spontaneous ovarian cancer
| Autor: | Besir Er, Fusun Erten, Kazim Sahin, Pınar Demirel Öner, Engin Yenice, Patrick Brice Defo Deeh, Cemal Orhan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_specialty Genistein Article Proinflammatory cytokine chemistry.chemical_compound Glycogen Synthase Kinase 3 GSK-3 Internal medicine Medicine Animals Protein kinase B Inflammation Ovarian Neoplasms IL-6 biology business.industry laying hens General Medicine medicine.disease Vascular endothelial growth factor Insulin receptor Disease Models Animal Endocrinology ovarian cancer chemistry TNF-α biology.protein Tumor necrosis factor alpha Female business Ovarian cancer Chickens Biomarkers |
| Zdroj: | Turkish Journal of Medical Sciences |
| ISSN: | 1303-6165 1300-0144 |
| Popis: | Background/aim Numerous studies show that cancer risk is reduced by consumption of soy-based foods containing genistein, but its effects on the glycogen synthase kinase-3 pathway (GSK-3) in ovarian cancer is unknown. Therefore, we tested the properties of genistein on inflammatory biomarkers and GSK-3 signaling pathways in the ovaries of old laying hens with ovarian cancer. Materials and methods A total of 300 laying hens were distributed into three groups as follows: group 1, animals fed a standard diet (comprising 22.39 mg of genistein/kg of diet); groups 2 and 3, animals fed a standard diet reconstituted with supplementation of 400 mg or 800 mg of genistein/kg of diet, respectively. Results Genistein modulated the inflammatory biomarkers by decreasing serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL- 6), interleukin-8 (IL-8), and vascular endothelial growth factor (VEGF) compared with control (p < 0.001). Moreover, it upregulated insulin receptor substrate-1 (p-IRS-1) and protein kinase B (p-AKT), but downregulated GSK-3α and β after treatment. It acts in a dose-dependent manner. Conclusion Genistein exhibited an anticancer effect by reducing proinflammatory biomarkers levels and inhibiting GSK-3 expression in the ovaries of old laying hens. It is a potential candidate in the chemoprevention and/or treatment of ovarian cancer. |
| Databáze: | OpenAIRE |
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