Scattered Regulatory Regions of the Chicken Immunoglobulin-.BETA. Gene and Two Adjacent Promoters of Ubiquitously Expressed Genes Interact with the Immunoglobulin-.BETA. Promoter in DT40 Cells
| Autor: | Masao Ono, Tomohiro Minbuta |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Pharmacology
Genetics Genes Immunoglobulin Transcription Genetic Response element Immunoglobulins Pharmaceutical Science E-box Promoter General Medicine TCF4 Regulatory Sequences Nucleic Acid Biology Chromatin Chromosomes Cell Line Epigenesis Genetic Epigenetics of physical exercise Regulatory sequence Animals Promoter Regions Genetic Chickens Gene ChIA-PET |
| Zdroj: | Biological and Pharmaceutical Bulletin. 34:1710-1716 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.34.1710 |
| Popis: | Recent studies indicate that several transcription units assemble to form a 'transcription factory' where active transcription occurs in the nuclei. Previously, we generated chicken B-lymphocyte-derived DT40 cells lacking six transcriptional regulatory regions scattered in and around the immunoglobulin (Ig)-β gene. The deletions caused a complete shut down of transcription and epigenetic regulation of the Ig-β gene, demonstrating that the scattered regulatory regions cooperated in the transcriptional and epigenetic regulation of the gene. However, the in vivo 3-dimensional spatial relationships between the Ig-β promoter and these six regulatory regions were not investigated. In this study, we used chromosome conformation capture (3C) technology and demonstrated that the Ig-β promoter physically interacted with the scattered regulatory regions. We found that the Ig-β promoter also interacted with two downstream promoters of ubiquitously expressed genes, rad motif 1 (RDM1) and Plekhm1, to form a transcription factory, but not with three ubiquitously expressed genes, BAF60b, p45/SUG, and RRMJ3, located upstream of the Ig-β gene. In this factory, the chromatin from the three promoters and the scattered regulatory regions of the Ig-β gene formed a complex structure with many chromatin loops. |
| Databáze: | OpenAIRE |
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