Prognostic Significance of microRNA-7 and its Roles in the Regulation of Cisplatin Resistance in Lung Adenocarcinoma
| Autor: | Mao-Wei Cheng, Gu-Yu Hu, Ze-Tian Shen, Li-Guo Luo |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Lung adenocarcinoma Male Lung Neoplasms Physiology Apoptosis Kaplan-Meier Estimate lcsh:Physiology 0302 clinical medicine lcsh:QD415-436 medicine.diagnostic_test lcsh:QP1-981 Middle Aged Prognosis Gene Expression Regulation Neoplastic 030220 oncology & carcinogenesis Lymphatic Metastasis Adenocarcinoma Female medicine.drug Adult medicine.medical_specialty Adenocarcinoma of Lung Disease-Free Survival Flow cytometry lcsh:Biochemistry 03 medical and health sciences Downregulation and upregulation Western blot Internal medicine microRNA medicine Biomarkers Tumor Humans Bcl-2 Chemosensitivity Aged Cisplatin Proportional hazards model business.industry miR-7 medicine.disease MicroRNA 7 MicroRNAs 030104 developmental biology Drug Resistance Neoplasm business |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 42, Iss 2, Pp 660-672 (2017) |
| ISSN: | 1421-9778 |
| Popis: | Background: Previously, microRNA (miR)-7 has been reported to function as a tumor suppressor in human cancers, but the correlations of miR-7 expression with prognosis and cisplatin (CDDP) resistance in lung adenocarcinoma (LA) are unclear. Here, our aim is to determine the prognostic significance of miR-7 and its roles in the regulation of CDDP resistance in LA. Methods: Quantitative real-time PCR (qRT-PCR) assay was performed to determine miR-7 expression in 108 paired of LA tissues and analyze its correlations with clinicopathological factors of patients. The patient survival data were collected retrospectively by Kaplan-Meier analyses, and multivariate analysis was performed using the Cox proportional hazards model to determine the prognostic significance of miR-7 expression. The effects of miR-7 expression on the chemosensitivity of LA cells to CDDP and its possible mechanisms were evaluated by MTT, flow cytometry, Western blot and luciferase assays. Results: It was observed that the relative expression level of miR-7 in LA tissues was significantly lower than that in the adjacent normal tissues and low miR-7 expression level was closely associated with poorer tumor differentiation, advanced pathological T-factor, higher incidence of lymph node metastasis and advanced p-TNM stage. Also, patients with low miR-7 expression showed a shorter overall survival than those with high miR-7 expression, and multivariate analysis indicated that status of miR-7 expression was an independent molecular biomarker for predicting the overall survival (OS) of LA patients. In addition, upregulation of miR-7 increases the sensitivity of LA cells to CDDP via induction of apoptosis by targeting Bcl-2. Conclusions: Our finding for the first time demonstrates that low miR-7 expression may be an independent poor prognostic factor and targeting miR-7 may be a potential strategy for the reversal of CDDP resistance in LA. |
| Databáze: | OpenAIRE |
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