Disulphates of 16-oxygenated ketonic c19 steroids as biliary metabolites of androsterone sulphate in female rats

Autor:	Hakozaki Misako, Matsui Michio
Rok vydání:	1978
Předmět:	Pharmacology Chromatography Androsterone Chemistry Organic Chemistry Clinical Biochemistry Fraction (chemistry) Mass spectrometry Biochemistry Chemical synthesis Rats chemistry.chemical_compound Endocrinology Sephadex Animals Bile Female Solvolysis Molecular Biology
Zdroj:	Steroids. 31:219-226
ISSN:	0039-128X
DOI:	10.1016/0039-128x(78)90114-9
Popis:	The chemical synthesis of 16beta-hydroxyandrosterone was described preparatory to studies of the disulphates of the 16-oxygenated ketonic C19 steroids present in the bile of female rats dosed with [3H]androsterone sulphate. The biliary metabolites were separated by chromatography on Sephadex LH-20 to afford monosulphate and diconjugate fractions. After solvolysis of the diconjugate fraction, the liberated steroids were separated by partition chromatography on Celite 545 and analyzed by gas chromatography-mass spectometry. In addition to 3alpha, 17beta-dihydroxy-5alpha-androstan-16-one isolated previously, 16beta-hydroxyandrosterone was identified as a disulphate.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6954aa34a70160134192db673242503f https://doi.org/10.1016/0039-128x(78)90114-9 Zobrazit plný text záznamu