Molecular cloning of a new human insulin-like growth factor binding protein
| Autor: | Richard S. Ioh, Diane M. Bauer, Michael C. Kiefer, Jürgen Zapf |
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| Rok vydání: | 1991 |
| Předmět: |
Molecular Sequence Data
Restriction Mapping Biophysics Receptors Cell Surface Molecular cloning Biology Biochemistry Polymerase Chain Reaction Rapid amplification of cDNA ends Somatomedins Complementary DNA Sequence Homology Nucleic Acid Humans Amino Acid Sequence RNA Neoplasm Cloning Molecular Molecular Biology Peptide sequence Gene Library chemistry.chemical_classification Osteosarcoma Base Sequence cDNA library Binding protein Nucleic acid sequence Receptors Somatomedin Cell Biology DNA Neoplasm Molecular biology Amino acid chemistry Oligonucleotide Probes |
| Zdroj: | Biochemical and biophysical research communications. 176(1) |
| ISSN: | 0006-291X |
| Popis: | We have cloned a new insulin-like growth factors binding protein (IGFBP) from a human osteosarcoma cDNA library. Two conserved regions in the COOH-terminal third of the five known human IGFBPs were used to design primers and to perform polymerase chain reaction (PCR) with osteosarcoma cDNA as a template. One of the eight PCR products encoded a unique IGFBP sequence. The DNA sequence was used to synthesize probes to screen an osteosarcoma cDNA library and isolate full length cDNA clones. The amino acid sequence was deduced from one of them. It contains two possible signal peptidase cleavage sites yielding a mature molecule of 257 or 252 amino acids, and 18 cysteines in identical positions to the other IGFBPs. The most pronounced homology exists with human IGFBP-3 (50 % in the NH 2− and 45 % in the COOH-terminal region). |
| Databáze: | OpenAIRE |
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