The serpin Spn5 is essential for wing expansion in Drosophila melanogaster
| Autor: | Rime Madani, Yves Charron, Jean-Dominique Vassalli, Vincent Gajdosik, Yeukuang Hwu, Giorgio Margaritondo, Chantal Combepine |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Embryology
Embryo Nonmammalian medicine.medical_treatment Mutant Expression Serpins/genetics/isolation & purification/metabolism Gene CIBM-PC RNA interference Drosophila Proteins Wings Animal ddc:576.5 Genome-Wide Analysis In Situ Hybridization Inhibition biology serpin Gene Expression Regulation Developmental Wing/metabolism Cell biology Drosophila melanogaster Oligonucleotide Microarrays Spn5 Recombinant Fusion Proteins/isolation & purification/metabolism Drosophila RNA Interference Proprotein Convertases Drosophila Proteins/genetics/isolation & purification/metabolism Plasmids animal structures Recombinant Fusion Proteins embryo Activation Serpin Drosophila melanogaster/embryology/genetics/growth & development/metabolism Escherichia coli medicine Animals Northern blot Psychological repression Serpins Protease Protein biology.organism_classification Blotting Northern Molecular biology Escherichia coli/genetics wing expansion Axis Formation Developmental Biology |
| Zdroj: | International Journal of Developmental Biology, Vol. 52, No 7 (2008) pp. 933-942 |
| ISSN: | 0214-6282 |
| Popis: | Serpins, a superfamily of protease inhibitors, control proteolytic cascades in many physiological processes. Genomic studies have revealed the presence of a high number of serpin-encoding genes in Drosophila melanogaster, but their functions remain largely unknown. In a biochemical screen designed to detect protease inhibitors that may be implicated in early Drosophila development, we identified in embryos a ligand that forms a 67 kDa SDS-stable complex with the broad spectrum protease trypsin. Characterization of this ligand revealed it to be the recently described serpin, Spn5. Expression analysis by in situ and Northern blot hybridization indicated maternal transmission of the transcript as well as zygotic expression in many larval, pupal and adult tissues. Targeted repression by RNA interference did not alter early embryogenesis but resulted in a complete defect in the unfolding and expansion of the wings of freshly eclosed mutant flies, without other detectable effects on development. |
| Databáze: | OpenAIRE |
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