Mass spectrometry of carbohydrate-protein interactions on a glycan array conjugated to CVD graphene surfaces
| Autor: | Alba Centeno, Amaia Zurutuza, Javier Calvo, Maurizio Prato, Alejandro Criado, Ilargi Napal, Sonia Serna, Niels C. Reichardt, Juan Pedro Merino |
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| Přispěvatelé: | Merino, J. P., Serna, S., Criado, A., Centeno, A., Napal, I., Calvo, J., Zurutuza, A., Reichardt, N., Prato, M. |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
grapheme 02 engineering and technology Conjugated system 010402 general chemistry Mass spectrometry LDI 01 natural sciences law.invention Protein–protein interaction carbohydrate microarray law General Materials Science carbohydrate microarrays chemical modification CVD lectin mass spectrometry biology Graphene Mechanical Engineering Lectin Chemical modification General Chemistry Carbohydrate 021001 nanoscience & nanotechnology Condensed Matter Physics Combinatorial chemistry 0104 chemical sciences Surface coating Mechanics of Materials biology.protein 0210 nano-technology |
| Zdroj: | 2D Materials |
| ISSN: | 2053-1583 |
| Popis: | 2D Materials ACCEPTED MANUSCRIPT • The following article is Open access Mass spectrometry of carbohydrate-protein interactions on a glycan array conjugated to CVD graphene surfaces Juan Pedro Merino1, Sonia Serna2 , Alejandro Criado3, Alba Centeno4 , Ilargi Napal5, Javier Calvo1, Amaia Zurutuza6, Niels Reichardt1 and Maurizio Prato7 Accepted Manuscript online 11 December 2019 • © 2019 IOP Publishing Ltd What is an Accepted Manuscript? Download Accepted Manuscript PDF Turn on MathJax Share this article Article information Abstract Mass spectrometry (MS) is a valuable tool for functional genomic, proteomic, and glycomic studies. Particularly, the combination of MS with microarrays is a powerful technique for analyzing the activity of carbohydrate processing enzymes and for the identification of carbohydrate-binding proteins (lectins) in complex matrices. On the other hand; graphene exhibits high desorption/ionization efficiency, good conductivity and optical transparency, specifications of a high-performance component for matrix-assisted laser desorption/ionization (MALDI) platforms. Besides, the chemical functionalization of graphene increases the adsorption capability of functional biomolecules (e.g. receptors), resulting in very stable interfaces. Taking advantage of the properties of graphene, we developed several modified chemical vapor deposited graphene (CVDG)-based glycan arrays on different substrates including ITO and bare glass, as a potential sensing platform for carbohydrate-lectin interactions, which are involved in a plethora of biological processes The glycan arrays were fully characterized by MALDI-MS analysis and, in some cases, optical microscopy. |
| Databáze: | OpenAIRE |
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