Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis
| Autor: | Asma Al Menhali, Ifrah I. Ali, Sayed A.M. Marzouk, Sherif M. Karam, Iltaf Shah |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
vitamin D3
0301 basic medicine Vitamin medicine.medical_specialty QH301-705.5 Biology Calcitriol receptor Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine Vitamin D and neurology vitamin D receptor Biology (General) Antrum Gastrin Parietal cell General Immunology and Microbiology digestive oral and skin physiology cell differentiation cell proliferation 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Immunohistochemistry gastric epithelial homeostasis General Agricultural and Biological Sciences Hormone |
| Zdroj: | Biology, Vol 10, Iss 705, p 705 (2021) Biology Volume 10 Issue 8 |
| ISSN: | 2079-7737 |
| DOI: | 10.3390/biology10080705 |
| Popis: | Simple Summary The gastric epithelium comprises multiple cell types that undergo continuous renewal through controlled proliferation, differentiation and death. Several vitamins, such as vitamin D (VD), are known to contribute to tissue homeostasis and numerous studies have shown the importance of VD in different body organ systems. However, VD’s normal function in the stomach is understudied. To better understand the role of VD in the murine stomach, we initially confirmed the expression of VD receptors (VDR) in the stomach. Mice were fed a VD-deficient diet for 3 months. The results showed that the proton pump of acid-secreting parietal cells was downregulated in vitamin D-deficient mice and contributed to an abnormal gastric physiology. Moreover, this diet increased the gastrin hormone gene expression and increased gastric epithelial cell proliferation. These findings suggest essential biological roles for VDR and VD in gastric epithelial homeostasis. Future research will be required to explore this phenomenon in humans. Abstract Unlike other organs, the importance of VD in a normal stomach is unknown. This study focuses on understanding the physiological role of vitamin D in gastric epithelial homeostasis. C57BL/6J mice were divided into three groups that were either fed a standard diet and kept in normal light/dark cycles (SDL), fed a standard diet but kept in the dark (SDD) or fed a vitamin D-deficient diet and kept in the dark (VDD). After 3 months, sera were collected to measure vitamin D levels by LC-MS/MS, gastric tissues were collected for immunohistochemical and gene expression analyses and gastric contents were collected to measure acid levels. The VDD group showed a significant decrease in the acid-secreting parietal cell-specific genes Atp4a and Atp4b when compared with the controls. This reduction was associated with an increased expression of an antral gastrin hormone. VDD gastric tissues also showed a high proliferation rate compared with SDL and SDD using an anti-BrdU antibody. This study indicates the requirement for normal vitamin D levels for proper parietal cell functions. |
| Databáze: | OpenAIRE |
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