Engineering of Biocompatible Coacervate-Based Synthetic Cells
| Autor: | Jan C. M. van Hest, Laurynas Vasiukas, Alexander F. Mason, N. Amy Yewdall, Marleen H. M. E. van Stevendaal |
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| Přispěvatelé: | Bio-Organic Chemistry, Institute for Complex Molecular Systems, ICMS Business Operations |
| Rok vydání: | 2021 |
| Předmět: |
Polymers
Amylose/chemical synthesis Biocompatible Materials 02 engineering and technology Biocompatible Materials/chemical synthesis 01 natural sciences Mice complex coacervates Materials Testing General Materials Science Polymers/chemical synthesis Cell Engineering Cells Cultured chemistry.chemical_classification Cultured Coacervate Molecular Structure Polymer synthetic cells protocells self-assembly 021001 nanoscience & nanotechnology block copolymers 0210 nano-technology Physical Organic Chemistry Cell Survival/drug effects Research Article Protocell polycations Materials science Artificial Cells/chemistry Biocompatibility Cell Survival Surface Properties Cells Nanotechnology 010402 general chemistry biocompatibility Animals Humans Viability assay Semipermeable membrane Particle Size Artificial cell 0104 chemical sciences RAW 264.7 Cells chemistry Artificial Cells Self-assembly Amylose HeLa Cells |
| Zdroj: | ACS Applied Materials & Interfaces Acs Applied Materials & Interfaces., 13, 7, pp. 7879-7889 Acs Applied Materials & Interfaces., 13, 7879-7889 ACS Applied Materials and Interfaces, 13(7), 7879-7889. American Chemical Society |
| ISSN: | 1944-8244 |
| Popis: | Polymer-stabilized complex coacervate microdroplets have emerged as a robust platform for synthetic cell research. Their unique core-shell properties enable the sequestration of high concentrations of biologically relevant macromolecules and their subsequent release through the semipermeable membrane. These unique properties render the synthetic cell platform highly suitable for a range of biomedical applications, as long as its biocompatibility upon interaction with biological cells is ensured. The purpose of this study is to investigate how the structure and formulation of these coacervate-based synthetic cells impact the viability of several different cell lines. Through careful examination of the individual synthetic cell components, it became evident that the presence of free polycation and membrane-forming polymer had to be prevented to ensure cell viability. After closely examining the structure-toxicity relationship, a set of conditions could be found whereby no detrimental effects were observed, when the artificial cells were cocultured with RAW264.7 cells. This opens up a range of possibilities to use this modular system for biomedical applications and creates design rules for the next generation of coacervate-based, biomedically relevant particles. |
| Databáze: | OpenAIRE |
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