Ectopic expression of maize polyamine oxidase and pea copper amine oxidase in the cell wall of tobacco plants

Autor: Giuseppina Rea, Paola Ferrante, Maria Concetta de Pinto, Valentina Gobbi, Rodolfo Federico, Stefania Biondi, Raffaela Tavazza, Laura De Gara, Riccardo Angelini, Paraskevi Tavladoraki
Přispěvatelé: REA G, DE PINTO MC, TAVAZZA R, BIONDI S., GOBBI V, FERRANTE P, DE GARA L, FEDERICO R, ANGELINI R, TAVALDORAKI P, Rea, G, DE PINTO M., C, Tavazza, R, Biondi, S, Gobbi, V, Ferrante, P, DE GARA, L, Federico, R, Angelini, R, Tavladoraki, Paraskevi, Rea, G., DE PINTO, M. C., Tavazza, R., Biondi, S., Gobbi, V., Ferrante, P., DE GARA, L., Federico, R., Angelini, Riccardo, Federico, Rodolfo, Angelini, R.
Rok vydání: 2004
Předmět:
Hypersensitive response
DIAMINE OXIDASE
DNA
Complementary
Physiology
Transgene
Nicotiana tabacum
pea
HYDROGEN-PEROXIDE
COPPER AMINE OXIDASE
GENE-EXPRESSION
SUBCELLULAR-LOCALIZATION
HYPERSENSITIVE RESPONSE
SUSPENSION-CULTURES
OXIDATIVE STRESS
POLYAMINE OXIDASE
hydrogen peroxide
Plant Science
POLYAMINE OXIDASE
Biology
maize
Lignin
Zea mays
copper amine oxidase
Gene Expression Regulation
Enzymologic
chemistry.chemical_compound
Cell Wall
Gene Expression Regulation
Plant
Tobacco
Genetics
Extracellular
Polyamines
Cloning
Molecular
Oxidase test
Oxidoreductases Acting on CH-NH Group Donors
fungi
Peas
food and beverages
Hydrogen Peroxide
Sequence Analysis
DNA
Plants
biology.organism_classification
Plants
Genetically Modified
GENETIC ENGINEERING
polyamine oxidase
Polyamine Catabolism
chemistry
Biochemistry
Amine Oxidase (Copper-Containing)
Polyamine
Polyamine oxidase
Oxidation-Reduction
Research Article
Zdroj: Plant physiology (Bethesda) 134 (2004): 1414–1426. doi:10.1104/pp.103.036764
info:cnr-pdr/source/autori:Rea G, de Pinto MC, Tavazza R, Biondi S, Gobbi V, Ferrante P, De Gara L, Federico R, Angelini R, Tavladoraki P./titolo:Ectopic expression of maize polyamine oxidase and pea copper amine oxidase in the cell wall of tobacco plants/doi:10.1104%2Fpp.103.036764/rivista:Plant physiology (Bethesda)/anno:2004/pagina_da:1414/pagina_a:1426/intervallo_pagine:1414–1426/volume:134
ISSN: 0032-0889
DOI: 10.1104/pp.103.036764
Popis: To test the feasibility of altering polyamine levels by influencing their catabolic pathway, we obtained transgenic tobacco (Nicotiana tabacum) plants constitutively expressing either maize (Zea mays) polyamine oxidase (MPAO) or pea (Pisum sativum) copper amine oxidase (PCuAO), two extracellular and H2O2-producing enzymes. Despite the high expression levels of the transgenes in the extracellular space, the amount of free polyamines in the homozygous transgenic plants was similar to that in the wild-type ones, suggesting either a tight regulation of polyamine levels or a different compartmentalization of the two recombinant proteins and the bulk amount of endogenous polyamines. Furthermore, no change in lignification levels and plant morphology was observed in the transgenic plants compared to untransformed plants, while a small but significant change in reactive oxygen species-scavenging capacity was verified. Both the MPAO and the PCuAO tobacco transgenic plants produced high amounts of H2O2 only in the presence of exogenously added enzyme substrates. These observations provided evidence for the limiting amount of freely available polyamines in the extracellular space in tobacco plants under physiological conditions, which was further confirmed for untransformed maize and pea plants. The amount of H2O2 produced by exogenously added polyamines in cell suspensions from the MPAO transgenic plants was sufficient to induce programmed cell death, which was sensitive to catalase treatment and required gene expression and caspase-like activity. The MPAO and PCuAO transgenic plants represent excellent tools to study polyamine secretion and conjugation in the extracellular space, as well as to determine when and how polyamine catabolism actually intervenes both in cell wall development and in response to stress.
Databáze: OpenAIRE
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