Modulation of inflammation by kininogen deficiency in a rat model of inflammatory arthritis
| Autor: | Irma Isordia-Salas, Robert W. Colman, Robin A. Pixley, Raul A. DeLa Cadena, Julian L. Castaneda, Albert Adam, Irma M. Sainz, Alexis Agelan, Bo Liu, R. Balfour Sartor |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Kininogen High-Molecular-Weight Kallikrein-Kinin System High-molecular-weight kininogen medicine.medical_treatment Inflammatory arthritis Immunology Intraperitoneal injection Arthritis Inflammation Peptidoglycan Hindlimb Rheumatology Polysaccharides Rats Inbred BN Internal medicine medicine Animals Immunology and Allergy Pharmacology (medical) Kininogen Foot business.industry Cartilage Body Weight medicine.disease Arthritis Experimental Rats Drug Combinations medicine.anatomical_structure Endocrinology Rats Inbred Lew Joints medicine.symptom business Injections Intraperitoneal |
| Zdroj: | Arthritis & Rheumatism. 52:2549-2552 |
| ISSN: | 1529-0131 0004-3591 |
| DOI: | 10.1002/art.21202 |
| Popis: | Objective To compare inflammatory peripheral arthritis in wild-type and high molecular weight kininogen (HK)–deficient rats, both on the genetically susceptible Lewis background. Methods By backcrossing Brown-Norway HK-deficient rats with Lewis rats for 6 generations, 2 new strains were produced, wild-type F6 and HK-deficient F6, each with a 98.5% Lewis genome. Inflammatory arthritis was induced by intraperitoneal injection of peptidoglycan–polysaccharide (PG-PS), and the clinical, histopathologic, and biochemical responses were compared in both strains. Results Eighteen days after PG-PS injection, rats with normal concentrations of HK showed weight loss and marked increase in hind ankle diameter with severe synovial inflammation and cartilage abnormalities. In contrast, HK-deficient rats showed no weight loss (P < 0.05), no increase in hind ankle diameter (P < 0.05), and an absence of inflammatory changes (P < 0.05), as measured by the histologic and morphometric Mankin grading system for synovial and cartilage injury. Conclusion Plasma HK is a key mediator of acute and chronic inflammatory arthritis in genetically susceptible Lewis rats. |
| Databáze: | OpenAIRE |
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