Quantifying the efficiency of Hydroxyapatite Mineralising Peptides
Autor: | Carole C. Perry, Robyn Plowright, David J. Belton, David L. Kaplan |
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Rok vydání: | 2017 |
Předmět: |
Science
Mineralogy chemistry.chemical_element Hydroxyapatite binding Peptide 02 engineering and technology Calcium 010402 general chemistry 01 natural sciences Article chemistry.chemical_compound Calcification Physiologic X-Ray Diffraction Spectroscopy Fourier Transform Infrared Brushite chemistry.chemical_classification Multidisciplinary Molecular Structure Chemistry Biomolecule 021001 nanoscience & nanotechnology Phosphate Trace Elements 0104 chemical sciences Concentration dependent Durapatite Reagent Medicine Peptides 0210 nano-technology Nuclear chemistry |
Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | We present a non-destructive analytical calibration tool to allow quantitative assessment of individual calcium phosphates such as hydroxyapatite (HAP) from mixtures including brushite. Many experimental approaches are used to evaluate the mineralising capabilities of biomolecules including peptides. However, it is difficult to quantitatively compare the efficacy of peptides in the promotion of mineralisation when inseparable mixtures of different minerals are produced. To address this challenge, a series of hydroxyapatite and brushite mixtures were produced as a percent/weight (0–100%) from pure components and multiple (N = 10) XRD patterns were collected for each mixture. A linear relationship between the ratio of selected peak heights and the molar ratio was found. Using this method, the mineralising capabilities of three known hydroxyapatite binding peptides, CaP(S) STLPIPHEFSRE, CaP(V) VTKHLNQISQSY and CaP(H) SVSVGMKPSPRP, was compared. All three directed mineralisation towards hydroxyapatite in a peptide concentration dependent manner. CaP(V) was most effective at inducing hydroxyapatite formation at higher reagent levels (Ca2+ = 200 mM), as also seen with peptide-silk chimeric materials, whereas CaP(S) was most effective when lower concentrations of calcium (20 mM) and phosphate were used. The approach can be extended to investigate HAP mineralisation in the presence of any number of mineralisation promoters or inhibitors. |
Databáze: | OpenAIRE |
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