Association of Toll-like receptor 3 and Toll-like receptor 9 single-nucleotide polymorphisms with hepatitis C virus persistence among Egyptians
| Autor: | Imam Waked, Eman Rewisha, Walaa R. Allam, Zainab Zakaria, Ahmed Osman, Iman Galal, Sayed F. Abdelwahab, Shaimaa Hamdy, Mohamed Abdel-Samiee, Maha Sobhy, Mohamed Hashem |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Health Personnel Hepatitis C virus Remission Spontaneous Gene Expression chemical and pharmacologic phenomena Single-nucleotide polymorphism Hepacivirus Biology medicine.disease_cause Polymorphism Single Nucleotide 03 medical and health sciences Polymorphism (computer science) Virology Outpatients Genotype Odds Ratio medicine Humans Genetic Predisposition to Disease Lymphocytes Case-control study virus diseases TLR9 hemic and immune systems General Medicine Hepatitis C Hepatitis C Chronic Middle Aged Viral Load medicine.disease Immunity Innate Toll-Like Receptor 3 030104 developmental biology Case-Control Studies Toll-Like Receptor 9 RNA Viral Egypt Female Viral load |
| Zdroj: | Archives of Virology. 163:2433-2442 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | Toll-like receptors (TLRs) give the innate immune system a considerable specificity for a large range of pathogens. TLR3 detects dsRNA of viruses while TLR9 recognizes bacterial and viral unmethylated CpG motifs. This study examined whether there is a potential association between single-nucleotide polymorphisms (SNPs) in the TLR3.rs3775290 (c.1377C/T), TLR9.rs5743836 (-1237T→C) and TLR9.rs352140 (G2848A) genes and HCV infection among Egyptian patients and healthcare workers (HCWs). We enrolled 546 subjects (409 HCWs and 137 patients) divided into four groups: group 1 included 265 seronegative, aviremic subjects; group 2 included 25 seronegative, viremic subjects; group 3 included 87 subjects with spontaneously resolved HCV infection; and group 4 included 169 chronic HCV patients. All subjects were genotyped for TLR3.rs3775290, TLR9.rs5743836 and TLR9.rs352140 SNPs by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis. TLR3.rs3775290 “CC” genotype was associated with chronic HCV infection, where there was a significantly greater frequency of this genotype among chronic patients when compared to subjects with spontaneously resolved infection (63.9% vs. 51.9%; p = 0.033; OR = 1.639 and 95% CI = 0.94-2.84). However, this SNP did not correlate with the HCV RNA load among the chronic subjects (p > 0.05). There was no significant difference in TLR9.rs5743836 and TLR9.rs352140 genotype distribution between groups (p > 0.05). Lack of association between the three SNPs was found, as the three SNPs are located on two different chromosomes. In conclusion, the TLR3.rs3775290 “CC” genotype was associated with HCV chronicity, while the TLR9 gene may not play a major role in HCV infection. |
| Databáze: | OpenAIRE |
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