Delayed alveolar clearance of nanoparticles through control of coating composition and interaction with lung surfactant protein A

Autor: Noelia A-Gonzalez, Jesús Ruiz-Cabello, Hugo Groult, Andrés Hidalgo, Fernando Herranz, Juan Pellico, Susana Carregal-Romero, Ana Victoria Lechuga-Vieco, Olga Cañadas, Cristina Casals, Belén García-Fojeda
Přispěvatelé: LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2021
Předmět:
Materials science
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
[SDV]Life Sciences [q-bio]
Magnetic Resonance Imaging (MRI)
02 engineering and technology
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
03 medical and health sciences
chemistry.chemical_compound
Pulmonary surfactant
In vivo
Phosphatidylcholine
medicine
Humans
[CHIM]Chemical Sciences
Bovine serum albumin
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
Lung
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology
environment/Health
0303 health sciences
Biología molecular
Pulmonary Surfactant-Associated Protein A
biology
lung clearance kinetics
Serum Albumin
Bovine
pulmonary administration
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
021001 nanoscience & nanotechnology
surfactant proteins
Magnetic Resonance Imaging
3. Good health
Surfactant protein A
medicine.anatomical_structure
chemistry
Drug delivery
Systemic administration
Biophysics
biology.protein
Nanoparticles
[SDV.IB]Life Sciences [q-bio]/Bioengineering
0210 nano-technology
Micellar superparamagnetic iron oxide
Zdroj: E-Prints Complutense. Archivo Institucional de la UCM
instname
Materials Science and Engineering: C
Materials Science and Engineering: C, Elsevier, 2021, pp.112551. ⟨10.1016/j.msec.2021.112551⟩
Carregal-Romero, S, Groult, H, Cañadas, O, A-Gonzalez, N, Lechuga-Vieco, A V, García-Fojeda, B, Herranz, F, Pellico, J, Hidalgo, A, Casals, C & Ruiz-Cabello, J 2021, ' Delayed alveolar clearance of nanoparticles through control of coating composition and interaction with lung surfactant protein A ', Materials Science and Engineering C . https://doi.org/10.1016/j.msec.2021.112551
ISSN: 0928-4931
DOI: 10.1016/j.msec.2021.112551⟩
Popis: The coating composition of nanomedicines is one of the main features in determining the medicines' fate, clearance, and immunoresponse in the body. To highlight the coatings' impact in pulmonary administration, two micellar superparamagnetic iron oxide nanoparticles (SPION) were compared. These nanoparticles are similar in size and charge but have different coatings: either phosphatidylcholine (PC-SPION) or bovine serum albumin (BSA-SPION). The aim of the study was to increase the understanding of the nano-bio interaction with the cellular and non-cellular components of the lung and underline valuable coatings either for local lung-targeted drug delivery in theranostic application or patient-friendly route systemic administration. PC-SPION and BSA-SPION were deposited in the alveoli by in vivo instillation and, despite the complexity of imaging the lung, SPION were macroscopically visualized by MRI. Impressively, PC-SPION were retained within the lungs for at least a week, while BSA-SPION were cleared more rapidly. The different lung residence times were confirmed by histological analysis and supported by a flow cytometry analysis of the SPION interactions with different myeloid cell populations. To further comprehend the way in which these nanoformulations interact with lung components at the molecular level, we used fluorescence spectroscopy, turbidity measurements, and dynamic light scattering to evaluate the interactions of the two SPION with surfactant protein A (SP-A), a key protein in setting up the NP behavior in the alveolar fluid. We found that SP-A induced aggregation of PC-SPION, but not BSA-SPION, which likely caused PC-SPION retention in the lung without inducing inflammation. In conclusion, the two SPION show different outcomes from interaction with SP-A leading to distinctive fate in the lung. PC-SPION hold great promise as imaging and theranostic agents when prolonged pulmonary drug delivery is required.
Databáze: OpenAIRE
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