Tunable light and drug induced depletion of target proteins
| Autor: | Jack A. Bates, Michael D. Bartoschek, Wen Deng, Hai Wei, Barbara Conradt, Heinrich Leonhardt |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
DNA Repair Light General Physics and Astronomy Apoptosis law.invention DNA Ligase ATP 0302 clinical medicine Ubiquitin law lcsh:Science Cell Engineering Caenorhabditis elegans Regulation of gene expression Multidisciplinary biology Chemistry Biological techniques Gene Expression Regulation Developmental Lamin Type A Cell biology DNA-Binding Proteins DARPin Caspases Recombinant DNA DNA repair DNA damage Ubiquitin-Protein Ligases Science Cytological Techniques Green Fluorescent Proteins Protein degradation General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Proliferating Cell Nuclear Antigen Developmental biology Animals Humans Caenorhabditis elegans Proteins fungi General Chemistry biology.organism_classification 030104 developmental biology biology.protein lcsh:Q 030217 neurology & neurosurgery DNA Damage HeLa Cells Transcription Factors |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Biological processes in development and disease are controlled by the abundance, localization and modification of cellular proteins. We have developed versatile tools based on recombinant E3 ubiquitin ligases that are controlled by light or drug induced heterodimerization for nanobody or DARPin targeted depletion of endogenous proteins in cells and organisms. We use this rapid, tunable and reversible protein depletion for functional studies of essential proteins like PCNA in DNA repair and to investigate the role of CED-3 in apoptosis during Caenorhabditis elegans development. These independent tools can be combined for spatial and temporal depletion of different sets of proteins, can help to distinguish immediate cellular responses from long-term adaptation effects and can facilitate the exploration of complex networks. Methods to control protein degradation usually require prior genetic engineering of each specific protein of interest. Here the authors present a toolbox for targeted depletion of endogenous proteins in cells and organisms, which is based on light- and drug-controlled recombinant E3 ubiquitin ligases. |
| Databáze: | OpenAIRE |
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