Expression, purification and characterization of pectin methylesterase inhibitor from kiwi fruit in Escherichia coli
| Autor: | Xiaohong Mei, Zhengyuan Zhai, Ying Huang, Yunbo Luo, Ruoyu Li, Xinyi Huang, Yanling Hao, Sheng Yin |
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| Rok vydání: | 2008 |
| Předmět: |
food.ingredient
Pectin medicine.disease_cause Chromatography Affinity law.invention food law medicine Escherichia coli Demethylation Plant Proteins chemistry.chemical_classification Temperature food and beverages Inhibitor protein Hydrogen-Ion Concentration Fusion protein Recombinant Proteins Enzyme Biochemistry chemistry Fruit Recombinant DNA Electrophoresis Polyacrylamide Gel Target protein Biotechnology |
| Zdroj: | Protein expression and purification. 60(2) |
| ISSN: | 1096-0279 |
| Popis: | A significant problem in production of fruit juices for human consumption is auto-clarification, where enzyme catalyzes pectin demethylation resulting in loss of the ''natural" cloudy appearance of juices. To overcome this problem, a plant inhibitor protein which blocks the action of pectin methylesterase has been used. In this paper, expression of recombinant kiwi pectin methylesterase inhibitor (PMEI) was carried out in Escherichia coli, and the target protein was expressed in the form of inclusion bodies. The expression level reached 46% of total cell protein. Then the fusion protein was purified by nickel ion metal affinity chromatography, and the purity was finally up to 98%. After refolding in GSH/GSSG redox system, recombinant PMEI not only could efficiently inhibit PMEs from eight different plants, but could remain effective inhibitor activity in the pH 3.0-10.0 and 20-40 degrees C. Thus, recombinant PMEI has potential application in the production of fruit juices product industry. |
| Databáze: | OpenAIRE |
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