Significance of CD34/CD123 expression in detection of minimal residual disease in B-ACUTE lymphoblastic leukemia in children
| Autor: | Howyda M. Kamal, Magda A. Zeidan, Asmaa Esh, Deena A. El Shabrawy, Reeham H. Abdel Sattar |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Neoplasm Residual Adolescent Interleukin-3 Receptor alpha Subunit CD34 Antigens CD34 Biology Immunophenotyping 03 medical and health sciences 0302 clinical medicine Antigen Precursor B-Cell Lymphoblastic Leukemia-Lymphoma hemic and lymphatic diseases Induction therapy medicine Humans B Acute Lymphoblastic Leukemia Child Molecular Biology B-Lymphocytes Infant Induction Chemotherapy Cell Biology Hematology Flow Cytometry Minimal residual disease medicine.anatomical_structure Case-Control Studies Child Preschool 030220 oncology & carcinogenesis Immunology Molecular Medicine Female Interleukin-3 receptor Bone marrow Blast Crisis Leukemic Blasts 030215 immunology |
| Zdroj: | Blood Cells, Molecules, and Diseases. 59:113-118 |
| ISSN: | 1079-9796 |
| Popis: | Background: MRD is seen as the major cause of disease relapse. So, it gives important feedback about conventional treatment success and helps in selecting therapeutic alternatives. We aimed to compare the expression of CD34/CD123 on normal B-cell precursors in bone marrow (“hematogones”) and on leukemic blasts in B-acute lymphoblastic leukemias (B-ALL) pediatric cases by flowcytometric analysis. Our study conducted on 20 children as a control and 30 B-ALL children cases at diagnosis and after 28 days of induction therapy. We found that the less mature hematogones (dim CD45 +) that express CD34 lack CD123 expression, whereas the more mature hematogones (moderate CD45 +) lack CD34 but always express CD123. In contrast with this discordant pattern of CD34 and CD123 expression in hematogones, blasts in 24 of 30 cases (80%) of B-ALL showed concordant expression pattern of the 2 antigens: 63% (19 of 30) cases expressed both antigens, whereas 17% (5 of 30) expressed neither. Our study concluded that these distinct patterns of CD34/CD123 expression on hematogones (discordant) and B-ALL blasts (concordant) are useful in differentiating small populations of residual blasts from hematogones after induction therapy to detect MRD. |
| Databáze: | OpenAIRE |
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