Ultrastructural changes in the myocardium in the experimental poisoning of rabbits by calcinogenic plant Solanum malacoxylon
| Autor: | Denise Russowsky, Claudio S.L. Barros, S. S. Barros, Eliana Dias da Costa Marçolla |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty Necrosis Sarcoplasm chemistry.chemical_element Biology Calcium Intoxicação por plantas Calcification Plantas venenosas Calcificação medicine Myocyte Von Kossa stain lcsh:SF1-1100 General Veterinary Myocardium Anatomy medicine.disease Staining Coelhos Basophilic chemistry Poisonous plants Plant poisoning Rabbits lcsh:Animal culture medicine.symptom Miocárdio |
| Zdroj: | Brazilian Journal of Veterinary Research and Animal Science, Vol 34, Iss 1 (1997) Brazilian Journal of Veterinary Research and Animal Science; Vol. 34 Núm. 1 (1997); 21-25 Brazilian Journal of Veterinary Research and Animal Science; Vol. 34 No. 1 (1997); 21-25 Brazilian Journal of Veterinary Research and Animal Science; v. 34 n. 1 (1997); 21-25 Brazilian Journal of Veterinary Research and Animal Science; V. 34 N. 1 (1997); 21-25 Brazilian Journal of Veterinary Research and Animal Science Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
| ISSN: | 1678-4456 1413-9596 |
| Popis: | Six rabbits of both sexes, 3-months old, were given via gastric tube an aqueous extract of the plant Solanum malacoxylon, equivalent to 100 mg of dried leaves per kg of body weight. Two rabbits (controls) did not receive the extract and were killed at the end of the experiment. All others rabbits were killed at 48,72 and 120 hours after extract administration. Fragments of myocardium were sampled and processed for both light and electron microscopy. Most significant gross changes observed were whitish streaks in the myocardium, especially in the right atrium. On light microscopic examination, groups of muscle cells had swollen sarcoplasms with loss of striations, slight basophila and eventual sarcoplasmic vacuolizations. These groups of cells were usually surrounded by macrophages, mesenchymal cells and few neutrophils admixed with necrotic debris. There was a delicate granular basophilic material in the interstitium. Von Kossa staining revealed, at these sites, calcium deposits within the sarcoplasm of cardiac myocytes in the interstitium and,occasionally, around apparently non damaged myocytes. There was mineralization of the medial tunic of coronary arteries. On the EMexamination the following changes were observed: activation of cardiac myocytes and interstitial cells, calcium deposits in the interstitiumand in the sarcosplasm of cardiac myocytes, as well as in the cytoplasm of interstitial cells. In late stages there was necrosis of cardiac myocytes, with mineralization and cytolisis. Few neutrophils and macrophages, with phagocytized mineral necrotic debris were also observed at these sites. The morphological aspect of the myocardium suggests that necrosis and calcification result from the direct action of the 1,25(OH)2D3 present in the plant on the myocardial cells. No changes in the myocardium of control rabbits were found. Seis coelhos com cerca de três meses, de ambos os sexos, receberam via sonda gástrica, extrato aquoso de Solanum malacoxylon equivalante a 100 mg de folhas dessecadas por kg de peso vivo. Dois animais não receberam o extrato e serviram como testemunhas, sendo sacrificados ao final do experimento. Entre 48, 72 e 120 horas após o início do experimento, todos os outros coelhos foram sacrificados e fragmentos do miocárdio foram processados para microscopia óptica e eletrônica. As alterações macroscópicas mais importantes foram estrias esbranquiçadas no miocárdio, particularmente no átrio esquerdo. Em microscopia óptica, grupos de células musculares mostravam sarcoplasma tumefeito, com perda das estriações, discreta basofilia e eventualmente vacuolizaçõesdo sarcoplasma. Estas células geralmente estavam rodeadas por macrófagos, células mesenquimais e alguns polimorfonucleares neutrófilos de permeio com material necrótico. No interstício foi evidenciado material basofílico finamente granular. A reação de vonKossa nestes locais revelou depósitos de cálcio no sarcoplasma dos míócitos, no interstício e, eventualmente, ao redor de células musculares aparentemente não lesadas. Os vasos coronários exibiam mineralização da média. Na ultra-estrutura foram observadas: ativação dos cardiomiócitos e das células intersticiais, deposição do cálcio no interstício e no sarcoplasma das células musculares cardíacas e no citoplasma de células intersticiais. Em fases mais avançadas observou-se nécrosé dos cardiomiócitos, com mineralização das células e lise celular. Alguns polimorfonucleares neutrófilos e macrófagos fagocitando material necrótico mineralizado são também vistos nestes locais. O aspecto morfológico do miocárdio surgere que as lesões de nécrosé e calcificação são o resultado da ação direta do 1,25(OH)2D3 contido na planta sobre as células do miocárdio. Os animais testemunhas não exibiram alterações no miocárdio. |
| Databáze: | OpenAIRE |
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