Arginine kinase isoforms in the closest protozoan relative of metazoans
| Autor: | W. Ross Ellington, Gregg G. Hoffman |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Signal peptide
DNA Complementary Physiology Molecular Sequence Data Protozoan Proteins Gene Expression Peptide Biochemistry Evolution Molecular Complementary DNA Genetics Protein Isoforms Amino Acid Sequence Choanoflagellate Molecular Biology Gene Choanoflagellata Phylogeny chemistry.chemical_classification Cloning biology Arginine Kinase Arginine kinase biology.organism_classification Molecular biology Amino acid chemistry biology.protein |
| Zdroj: | Comparative Biochemistry and Physiology Part D: Genomics and Proteomics. 6:171-177 |
| ISSN: | 1744-117X |
| Popis: | The genome of the choanoflagellate Monosiga brevicollis contains at least three genes for the phosphoryl transfer enzyme, arginine kinase (AK; EC 2.7.3.3). Bioinformatic analyses of the deduced amino acid sequences of the proteins coded for by two of these genes showed that one of these AKs is cytoplasmic (denoted AK1) while the other appears to have an N-terminal mitochondrial targeting peptide (denoted AK2). Cloning and expression of the cDNA for AK1 yielded considerable soluble AK activity. Three AK2 constructs were expressed - one corresponding to the full length protein and two corresponding to truncated versions in which the signal peptide had been deleted. Expression of the former construct yielded minimal soluble activity. In contrast, significant AK activity was found in both truncated constructs confirming the importance of removal of the targeting peptide for proper folding and catalytic activity. Both AK1 and AK2 are functional oligomers unlike typical AKs which are monomeric. A phylogenetic analysis showed that these choanoflagellate AKs group more closely with a supercluster consisting of cytoplasmic and mitochondrial CKs and invertebrate AKs that evolved secondarily from a CK-like ancestor. Reaction-diffusion constraints in choanoflagellates are likely mitigated by the presence of AK isoforms which facilitate energy transport in these highly polarized cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect