Mycobacterium bovis strain bacillus Calmette-Guérin-induced liver granulomas contain a diverse TCR repertoire, but a monoclonal T cell population is sufficient for protective granuloma formation
| Autor: | Laura H. Hogan, Irena Malkovska, Glenn J. Fennelly, Matyas Sandor, Khen Macvilay, Brittany Barger, Dominic O. Co |
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| Rok vydání: | 2001 |
| Předmět: |
T cell
Immunology Population Receptors Antigen T-Cell Epitopes T-Lymphocyte Mice Transgenic Lymphocyte Activation Microbiology Immunophenotyping Mice Cell Movement T-Lymphocyte Subsets hemic and lymphatic diseases medicine Immunology and Allergy Cytotoxic T cell Animals education Mycobacterium bovis education.field_of_study Mice Inbred C3H Mycobacterium Infections Granuloma biology T-cell receptor medicine.disease biology.organism_classification Virology Clone Cells Mice Inbred C57BL medicine.anatomical_structure Liver Monoclonal CD8 Spleen |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 166(10) |
| ISSN: | 0022-1767 |
| Popis: | Granuloma formation is a form of delayed-type hypersensitivity requiring CD4+ T cells. Granulomas control the growth and dissemination of pathogens, preventing host inflammation from harming surrounding tissues. Using a murine model of Mycobacterium bovis strain bacillus Calmette-Guérin (BCG) infection we studied the extent of T cell heterogeneity present in liver granulomas. We demonstrate that the TCR repertoire of granuloma-infiltrating T cells is very diverse even at the single-granuloma level, suggesting that before granuloma closure, a large number of different T cells are recruited to the lesion. At the same time, the TCR repertoire is selected, because AND TCR transgenic T cells (Vα11/Vβ3 anti-pigeon cytochrome c) are preferentially excluded from granulomas of BCG-infected AND mice, and cells expressing secondary endemic Vβ-chains are enriched among AND cells homing to granulomas. Next, we addressed whether TCR heterogeneity is required for effective granuloma formation. We infected 5CC7/recombinase-activating gene 2−/− mice with recombinant BCG that express pigeon cytochrome c peptide in a mycobacterial 19-kDa bacterial surface lipoprotein. A CD4+ T cell with a single specificity in the absence of CD8+ T cells is sufficient to form granulomas and adequately control bacteria. Our study shows that expanded monoclonal T cell populations can be protective in mycobacterial infection. |
| Databáze: | OpenAIRE |
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