Effect of 0.12% chlorhexidine in reducing microorganisms found in aerosol used for dental prophylaxis of patients submitted to fixed orthodontic treatment
Autor: | Myrela Galvão Cardoso Costa, Ana Cristina Azevedo Moreira, Isis Rodrigues Menezes dos Santos, Marcelo de Castellucci e Barbosa |
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Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Adult
Male Adolescent Air microbiology Air Microbiology Mouthwashes Dentistry Orthodontics Orthodontics Corrective Air contamination Aerosol propellant chemistry.chemical_compound Young Adult stomatognathic system Orthodontic Appliances medicine Humans Statistical analysis Longitudinal Studies Child Aerosolization Effects of air contamination Aerosols Sodium bicarbonate Bacteria business.industry Dental Prophylaxis Propelentes de aerossol Chlorhexidine Clorexidina Efeitos da contaminação do ar Bacterial Load lcsh:RK1-715 Sodium Bicarbonate chemistry lcsh:Dentistry Air Pollution Indoor Anti-Infective Agents Local Original Article Female Oral Surgery business medicine.drug |
Zdroj: | Dental Press Journal of Orthodontics Dental Press Journal of Orthodontics v.19 n.3 2014 Dental Press International (DPI) instacron:DPI Dental Press Journal of Orthodontics, Volume: 19, Issue: 3, Pages: 101-95, Published: JUN 2014 Dental Press Journal of Orthodontics, Vol 19, Iss 3, Pp 95-101 (2014) |
ISSN: | 2177-6709 2176-9451 |
Popis: | OBJECTIVE: This study aimed at assessing, in vivo, whether the prior use of 0.12% chlorhexidine as mouthwash would decrease air contamination caused by aerosolized sodium bicarbonate during dental prophylaxis. The study was conducted with 23 patients aged between 10 and 40 years old who were randomly selected and undergoing fixed orthodontic treatment. METHODS: The study was divided into two phases (T1 and T2) with a 30-day interval in between. In both phases, dental prophylaxis was performed with aerosolized sodium bicarbonate jetted to the upper and lower arches for 4 minutes. In T1, 10 minutes before the prophylaxis procedure, the participants used distilled water as mouthwash for one minute; whereas in T2, mouthwash was performed with 0.12% chlorhexidine. Microbial samples were collected in BHI agar plates for microbiological analysis. Two dishes were positioned on the clinician (10 cm from the mouth) and a third one at 15 cm from the patient's mouth. The samples were incubated for 48 hours at 37°C. Results were expressed in colony-forming units (CFU). RESULTS: Statistical analysis carried out by means of Student's t test, as well as Wilconxon and Kruskal-Wallis tests revealed that the prior use of 0.12% chlorhexidine as mouthwash significantly reduced CFU in the three positions studied (P < 0.001). CONCLUSION: The prior use of 0.12% chlorhexidine as mouthwash significantly reduced contamination caused by aerosolized sodium bicarbonate during dental prophylaxis in the orthodontic clinic. OBJETIVO: avaliar, in vivo, se a utilização prévia do bochecho com clorexidina a 0,12% diminui a contaminação do ar gerada pelo jato de bicarbonato de sódio durante a profilaxia dentária. O estudo foi realizado com 23 pacientes, na faixa etária entre 10 e 40 anos, escolhidos aleatoriamente, que faziam uso de aparelho ortodôntico fixo. MÉTODOS: o estudo foi dividido em duas fases (T1 e T2), com intervalo de 30 dias entre elas. Em ambas, foi realizada profilaxia dentária com jato de bicarbonato de sódio na arcada superior e inferior, durante quatro minutos. Em T1, 10 minutos antes do procedimento, os participantes realizaram bochecho com água destilada durante um minuto, e, em T2, o bochecho foi realizado com clorexidina a 0,12%. Amostras dos microrganismos foram coletadas em placas de ágar BHI para análise microbiológica, sendo duas placas posicionadas no profissional (a 10cm da boca) e uma terceira a 15cm da boca do paciente. Após a coleta, as placas foram incubadas por 48 horas a 37°C. O resultado foi expresso em número de unidades formadoras de colônias (UFC). RESULTADOS: após análise estatística utilizando teste t de Student, teste de Wilconxon e teste de Kruskal-Wallis, observou-se que o bochecho prévio com clorexidina a 0,12% reduziu significativamente a média de UFC nas três posições estudadas (p < 0,001). CONCLUSÃO: os resultados permitem concluir que o bochecho prévio com clorexidina a 0,12% proporcionou uma redução estatisticamente significativa na contaminação gerada por meio do jato de bicarbonato de sódio durante a profilaxia dentária na clínica ortodôntica. |
Databáze: | OpenAIRE |
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