The oxidation metabolites of endomorphin 1 and its fragments induced by free radicals
| Autor: | Qian-Yu Zhao, Fu Xin Chen, Rui Wang, Guo Fen Ma, Pin Gong |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Agonist Antioxidant Free Radicals medicine.drug_class Stereochemistry Radical medicine.medical_treatment Peptide Biochemistry chemistry.chemical_compound Structural Biology In vivo Drug Discovery medicine Animals Rats Wistar Molecular Biology Chromatography High Pressure Liquid Pharmacology chemistry.chemical_classification Superoxide Cell Membrane Organic Chemistry Electron Spin Resonance Spectroscopy Brain Endomorphin-1 General Medicine NAD Free radical scavenger Rats chemistry Molecular Medicine Oligopeptides Oxidation-Reduction |
| Zdroj: | Journal of Peptide Science. 15:337-344 |
| ISSN: | 1099-1387 1075-2617 |
| Popis: | Endomorphin 1 (EM1), an endogenous µ-opioid receptor agonist, acts as a free radical scavenger in vitro and an antioxidant in vivo. The modification of EM1 by ROS and the properties of the OM attracted our attention. In vitro assays were performed via RP-HPLC, spectrophotometric measurements, EPR and amino acid analysis, Schmorl's reaction to define the formation of melanin-like compounds transformed from EM1, collectively named EM1–melanin and by solubility assay, radioligand-binding assay, NADH oxidation, superoxide anion scavenging assay to study some physical and chemical properties of EM1–melanin. Possible pathways of the formation of EM1–melanin were proposed. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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