Photo-oxidative stress by ultraviolet-B radiation and antioxidative defense of eckstolonol in human keratinocytes
| Autor: | Bo-Ram Ye, Ji Hyung Kim, Jiyi Jang, Seunghee Han, Young-Ung Choi, Do-Hyung Kang, Kon-Tak Yoon, Zhong-Ji Qian, Soo-Jin Heo, Chulhong Oh, Il-Whan Choi, Abu Affan, Se Chang Park, Won-Kyo Jung |
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| Rok vydání: | 2012 |
| Předmět: |
Keratinocytes
Antioxidant Cell Survival Ultraviolet Rays Health Toxicology and Mutagenesis medicine.medical_treatment Phaeophyta Toxicology medicine.disease_cause Heterocyclic Compounds 4 or More Rings Antioxidants Cell Line Dioxanes Lipid peroxidation Superoxide dismutase chemistry.chemical_compound medicine Humans Viability assay Pharmacology chemistry.chemical_classification Reactive oxygen species biology Plant Extracts Superoxide Dismutase General Medicine Catalase Molecular biology Comet assay Oxidative Stress chemistry Biochemistry biology.protein Comet Assay Lipid Peroxidation Reactive Oxygen Species Oxidative stress |
| Zdroj: | Environmental Toxicology and Pharmacology. 34:926-934 |
| ISSN: | 1382-6689 |
| DOI: | 10.1016/j.etap.2012.08.003 |
| Popis: | Ultraviolet-B (UV-B) irradiation has been known to generate oxidative stress by increasing reactive oxygen species (ROS) in skin cells. Several naturally occurring antioxidant compounds isolated from marine algae are believed to protect against ROS. In this study, we assessed the antioxidative effect of eckstolonol isolated from Ecklonia cava against UV-B-induced ROS in human keratinocytes (HaCaTs). We investigated the effects of photo-oxidative stress by UV-B (50 mJ/cm(2)) and the antioxidative effects of eckstolonol using fluorometry, flow cytometry, microscopy, and cell viability and comet assays. UV-B irradiation decreased cell viability, which was restored in a dose-dependent manner with eckstolonol treatment (0, 5, 50, 100, and 200 μM). Moreover, eckstolonol reduced UV-B-induced ROS, lipid peroxidation, damaged DNA levels, and cell death. These antioxidative effects seem to be due to the enzymatic activities of catalase (CAT) and superoxide dismutase (SOD). Collectively, these results indicate that eckstolonol is capable of protecting keratinocytes from photo-oxidative stress. |
| Databáze: | OpenAIRE |
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