Biomarkers in exhaled breath condensate indicate presence and severity of cystic fibrosis in children
Autor: | Jan-Bart L. Yntema, Edward Dompeling, Hein J. L. Brackel, Philippe P.R. Rosias, Gertjan J.M. den Hartog, Will K. W. H. Wodzig, Jan Damoiseaux, Charlotte M. H. H. T. Robroeks, Quirijn Jöbsis, Dillys van Vliet |
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Přispěvatelé: | Kindergeneeskunde, Huisartsgeneeskunde, Interne Geneeskunde, Farmacologie & Toxicologie, MUMC+: DA CDL Algemeen (9), RS: NUTRIM - R3 - Chronic inflammatory disease and wasting, RS: NUTRIM - R4 - Gene-environment interaction, RS: CARIM School for Cardiovascular Diseases |
Rok vydání: | 2008 |
Předmět: |
Male
medicine.medical_specialty Adolescent Cystic Fibrosis Exacerbation Immunology Dinoprost Nitric Oxide Severity of Illness Index Cystic fibrosis Gastroenterology Nitric oxide chemistry.chemical_compound Internal medicine medicine Humans Immunology and Allergy Exhaled breath condensate Expiration Respiratory system Nitrite Child Nitrites Nitrates business.industry Hydrogen Peroxide medicine.disease Cross-Sectional Studies Logistic Models Genetic defects of metabolism [UMCN 5.1] Breath Tests chemistry Exhalation Pediatrics Perinatology and Child Health Exhaled nitric oxide Cytokines Female business Biomarkers |
Zdroj: | Pediatric Allergy and Immunology, 19, 652-9 Pediatric Allergy and Immunology, 19(7), 652-9. Wiley Pediatric Allergy and Immunology, 19, 7, pp. 652-9 |
ISSN: | 1399-3038 0905-6157 |
DOI: | 10.1111/j.1399-3038.2007.00693.x |
Popis: | Contains fulltext : 70193.pdf (Publisher’s version ) (Closed access) Chronic airway inflammation is present in cystic fibrosis (CF). Non-invasive inflammometry may be useful in disease management. The aim of the present cross-sectional study was to investigate: (i) the ability of fractional exhaled nitric oxide and inflammatory markers (IM) [exhaled breath condensate (EBC) acidity, nitrite, nitrate, hydrogen peroxide (H(2)O(2)), 8-isoprostane, Th1/Th2 cytokines] to indicate (exacerbations of) CF; and (ii) the ability of these non-invasive IM to indicate CF disease severity. In 98 children (48 CF/50 controls), exhaled nitric oxide was measured using the NIOX, and condensate was collected using a glass condenser. In CF interferon (IFN-gamma) and nitrite concentrations were significantly higher, whereas exhaled nitric oxide levels were significantly lower compared with controls (3.3 +/- 0.3 pg/ml, 2.2 +/- 0.2 microM, 10.0 +/- 1.2 p.p.b. vs. 2.6 +/- 0.2 pg/ml, 1.4 +/- 0.1 microM, 15.4 +/- 1.4 p.p.b. respectively). Using multivariate logistic regression models, the presence of CF was best indicated by 8-isoprostane, nitrite and IFN-gamma [sensitivity 78%, specificity 83%; area under receiver operating characteristic curve (AUC) 0.906, p < 0.001]. An exacerbation of CF was best indicated by 8-isoprostane and nitrite (sensitivity 40%, specificity 97%, AUC curve 0.838, p = 0.009). Most indicative biomarkers of CF severity were exhaled nitric oxide, and condensate acidity (sensitivity 96%, specificity 67%; AUC curve 0.751, p = 0.008). In this cross-sectional study, the combination of different exhaled IM could indicate (exacerbations of) CF, and severity of the disease in children. Longitudinal data are necessary to further confirm the role of these markers for the management of CF in children. |
Databáze: | OpenAIRE |
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