Iloprost Reduces Procoagulant Activity in the Extracorporeal Circuit
| Autor: | R. L. Korn, Jeevanandam, N. Stenach, Addonizio Vp, Carol A. Fisher |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Extracorporeal Circulation
Platelet Aggregation Pharmacology Platelet Factor 4 Fibrinogen Extracorporeal law.invention Leukocyte Count law Coagulopathy medicine Cardiopulmonary bypass Humans Platelet Iloprost Blood Coagulation Fibrinopeptide A Pancreatic Elastase Heparin Platelet Count Chemistry Extracorporeal circulation Platelet Activation beta-Thromboglobulin medicine.disease Anesthesia Surgery Leukocyte Elastase medicine.drug |
| Zdroj: | Journal of Surgical Research. 55:433-440 |
| ISSN: | 0022-4804 |
| Popis: | Although activation of formed blood elements during cardiopulmonary bypass has been examined, its presumed procoagulant role has not been identified or quantified. We evaluated the effects of iloprost, an inhibitor of platelet and leukocyte function, on subclinical coagulation during simulated extracorporeal circulation. We determined that a heparin dose of 1 U/ml prevented clot formation in this model, but resulted in elevated plasma levels of fibrinopeptide A, the first cleavage product of fibrinogen. Human blood was recirculated with 1 U/ml heparin using a roller pump and pediatric reversed hollow fiber oxygenator (0.8 m2) for 2 hr at 37 degrees C. Iloprost (1 ng/ml, n = 5) reduced platelet adhesion, with platelet counts of 78 +/- 7% (mean +/- SEM) of baseline during 2 hr of simulated extracorporeal circulation, compared to 36 +/- 6% in control circuits (CONT: n = 6, P0.05). Plasma levels of platelet factor 4 and beta-thromboglobulin were also reduced by iloprost (486 +/- 116 ng/ml vs CONT, 2933 +/- 275 ng/ml, P0.05, and 938 +/- 274 ng/ml vs CONT, 5700 +/- 1109 ng/ml, P0.05, respectively). Circulating leukocyte counts were maintained in iloprost circuits (6.4 +/- 0.6 x 10(3)/mm3 vs CONT, 4.2 +/- 0.3 x 10(3)/mm3, P0.05), and neutrophil elastase levels rose to only 0.4 +/- 0.1 ng/ml in iloprost circuits, compared to 0.8 +/- 0.1 ng/ml in CONT (P0.05). Finally, iloprost treatment reduced fibrinopeptide A levels to 102 +/- 28 ng/ml (CONT, 793 +/- 337 ng/ml, P0.05) after 2 hr.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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