Outbreak of H5N2 highly pathogenic avian Influenza A virus infection in two commercial layer facilities: lesions and viral antigen distribution
| Autor: | Paulo Arruda, Jianqiang Zhang, Karen Harmon, Pablo Piñeyro, Bailey L. Arruda, Rodger G. Main, Vickie L. Cooper, Darin M. Madson, Gregory W. Stevenson, Rachel J. Derscheid, Patrick G. Halbur, Mary Lea Killian, Kent Schwartz, Phillip C. Gauger, Eric R. Burrough, Kyoung-Jin Yoon, Yuko Sato, Drew R. Magstadt |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty 040301 veterinary sciences Spleen Biology medicine.disease_cause Real-Time Polymerase Chain Reaction Disease Outbreaks 0403 veterinary science Avian Influenza A Virus 03 medical and health sciences medicine Animals Antigens Viral Phylogeny Lung General Veterinary Outbreak 04 agricultural and veterinary sciences Virology Iowa Influenza A virus subtype H5N1 030104 developmental biology medicine.anatomical_structure Liver Influenza in Birds Respiratory epithelium Choroid plexus Pancreas Influenza A Virus H5N2 Subtype Chickens |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 28(5) |
| ISSN: | 1943-4936 |
| Popis: | The largest outbreak of highly pathogenic avian Influenza A virus (HPAIV) infection in U.S. history began in December 2014 resulting in the euthanasia of millions of birds and collateral economic consequences to the U.S. poultry industry. We describe 2 cases of H5N2 HPAIV infection in laying hens in Iowa. Following a sharp increase in mortality with minimal clinical signs, 15 dead birds, from 2 unrelated farms, were submitted to the Iowa State University Veterinary Diagnostic Laboratory. Common lesions included diffuse edema and multifocal hemorrhage of the comb, catarrhal exudate in the oropharynx, and multifocal tracheal hemorrhage. Less common lesions included epicardial petechiae, splenic hemorrhage, and pancreatic necrosis. Influenza A virus nucleoprotein was detected by immunohistochemistry in multiple cell types including ependymal cells, the choroid plexus, neurons, respiratory epithelium and macrophages in the lung, cardiac myocytes, endothelial cells, necrotic foci in the spleen, Kupffer cells in the liver, and necrotic acinar cells in the pancreas. Real-time polymerase chain reaction and sequencing confirmed H5N2 HPAIV with molecular characteristics similar to other contemporary U.S. H5N2 HPAIVs in both cases. |
| Databáze: | OpenAIRE |
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