Improved resolution of fluoroquinolones using cetyltrimethyl ammonium bromide-micellar electrokinetic chromatography and its application to residue analysis in surface water
| Autor: | Leena Suntornsuk, Brompoj Prutthiwanasan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Ammonium bromide
Analyte Clinical Biochemistry 010501 environmental sciences 01 natural sciences Biochemistry Micelle Micellar electrokinetic chromatography Analytical Chemistry chemistry.chemical_compound Rivers Limit of Detection Enrofloxacin medicine Solid phase extraction 0105 earth and related environmental sciences Chromatography Micellar Electrokinetic Capillary Detection limit Chromatography Cetrimonium 010401 analytical chemistry Reproducibility of Results Cell Biology General Medicine 0104 chemical sciences chemistry Cetrimonium Compounds Linear Models Ofloxacin Water Pollutants Chemical medicine.drug Fluoroquinolones |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1092 |
| ISSN: | 1873-376X |
| Popis: | A simple micellar electrokinetic chromatography (MEKC), using cetyltrimethyl ammonium bromide (CTAB) as micelles, for the determination of ciprofloxacin (CIP), enrofloxacin (ENR), norfloxacin (NOR) and ofloxacin (OFL) residues in surface water was developed. Peak Master was used for predicting amounts of analyte ionic forms to reduce numbers of tedious experiments in optimizing the analyte capacity factors. A baseline separation (Rs > 2.8) of the analytes was achieved in 7 min using 15 mM sodium dihydrogen phosphate (pH 6.0) containing 3 mM CTAB and a capillary with an effective length of 56.0 cm. A negative polarity voltage of 20 kV was used to enable the migration of the cationic micelles toward the detection site. The method shows good linearity in a range of 5 and 20 μg mL-1 and precision (%RSD < 6.45). Percent recoveries of the method were in a range of 65.1-88.7%. The limits of detection and quantitation were in the ranges of 1-2 and 3-5 μg mL-1, respectively. Two steps sample clean-up and preconcentration of surface water samples by hydrophilic-lipophilic balance and fluoroquinolone-molecularly imprinted polymer were advantageous for removal of humic acids and enabling the detection of fluoroquinolone residues in the samples. Finally, the method was applied for fluoroquinolone residues analysis of surface water in Thailand. |
| Databáze: | OpenAIRE |
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