Molecular characterisation of airport malaria : four cases in France during summer 1999
| Autor: | R. Durand, D. Lusina, J. Le Bras, Sayeh Jafari |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Drug Resistance Protozoan Proteins Plasmodium malariae Polymerase Chain Reaction Genotype Malaria Falciparum Merozoite surface protein Merozoite Surface Protein 1 Genetics Travel biology airport malaria Occupational Diseases Infectious Diseases Female Adult Paris Veterinary (miscellaneous) Plasmodium falciparum Antigens Protozoan Locus (genetics) lcsh:Infectious and parasitic diseases Antimalarials Anopheles parasitic diseases medicine Animals Humans lcsh:RC109-216 Codon Genotyping DNA Protozoan biology.organism_classification medicine.disease Virology Insect Vectors Malaria Protein Structure Tertiary Tetrahydrofolate Dehydrogenase genotyping Genetic marker Insect Science in vitro susceptibility Aerospace Medicine Animal Science and Zoology Parasitology |
| Zdroj: | Europe PubMed Central Parasite, Vol 9, Iss 2, Pp 187-191 (2002) |
| ISSN: | 1776-1042 1252-607X |
| DOI: | 10.1051/parasite/2002092187 |
| Popis: | Four airport malaria cases have been observed in the vicinity of the Roissy-Charles-de-Gaulle International Airport, Paris, France. These cases were geographically very close to each other and clustered in a short period of time during the summer of 1999. The phenotype and genotype of the Plasmodium falciparum isolates obtained from these patients were determined in order to know whether a single mosquito could have infected more than one subject. The genomic characterisation of isolates was performed using the polymorphic markers merozoite surface protein 1 (Msp 1) and merozoite surface protein 2 (Msp 2) genes, the kappa and omega repeats domains of cg2 and the dihydrofolate reductase (DHFR) genotypes. Results showed identical genotypes for isolates 1, 2 and 4 whereas the genotype of isolate 3 differed at one locus. The molecular analysis was consistent with the hypothesis that all patients could have been bitten by the same mosquito and that patient 3, may have received a different clone and an additional species. In vitro susceptibility data did not confirm or rule out this hypothesis because isolates had the same profile of susceptibility to the tested drugs. |
| Databáze: | OpenAIRE |
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