The effect of dizocilpine and enadoline on immediate early gene expression in the gerbil global ischaemia model
| Autor: | Geoffrey N. Woodruff, J.A. Poat, Valerie L. Woodburn, Neil J. Hayward, John Hughes |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_specialty
Pyrrolidines JUNB Hippocampus Gene Expression Biology Gerbil Neuroprotection Receptors N-Methyl-D-Aspartate Brain Ischemia Cellular and Molecular Neuroscience chemistry.chemical_compound Internal medicine medicine Animals RNA Messenger Genes Immediate-Early Heat-Shock Proteins Benzofurans Pharmacology Enadoline Dentate gyrus Receptors Opioid kappa Dizocilpine DNA-Binding Proteins Endocrinology nervous system chemistry Autoradiography Female Dizocilpine Maleate Gerbillinae Immediate early gene medicine.drug Transcription Factors |
| Zdroj: | Neuropharmacology. 32(10) |
| ISSN: | 0028-3908 |
| Popis: | Increases in mRNA levels for c-fos, c-jun, junB, hsp 70 and NGFI-A were observed in the dentate gyrus of the hippocampus following 7 min ischaemia in the Mongolian gerbil. The response was rapid and transient (30 min to 4hr) for NGFI-A, junB and c-fos mRNA. In contrast c-jun mRNA remained increased for several hours. Hsp 70 increased in the dentate gyrus 1 hr after the insult, returned to control values at 4 hr and showed a secondary increase at 24 hr. At 24 hr increased hsp 70 mRNA was observed in other regions of the CNS, i.e. CA1, CA2, CA3 and cortex. The non-competitive NMDA receptor antagonist, dizoclipine, attenuated the increases in IEG expression and was neuroprotective. In contrast the kappa opiate receptor agonist, enadoline, protected the CA1 neurones from degeneration but did not inhibit the increased levels of IEG mRNA. Increases in hsp 70 mRNA were reduced to baseline by both enadoline and dizocilpine. These results suggest that inhibition of IEG expression is not a prerequisite for neuroprotection. However, hsp 70 was predictive of neuronal protection and may be a useful assay in this and related models. |
| Databáze: | OpenAIRE |
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