Bisphenol A induces spermatocyte apoptosis in rare minnow Gobiocypris rarus

Autor: Mengqian Cheng, Lang Wu, Yingying Zhang, Zaizhao Wang, Guo Zhang
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
endocrine system
Health
Toxicology and Mutagenesis
Blotting
Western
Cyprinidae
Apoptosis
Spermatocyte
010501 environmental sciences
Aquatic Science
Biology
Endocrine Disruptors
Real-Time Polymerase Chain Reaction
01 natural sciences
Andrology
03 medical and health sciences
Western blot
Meiosis
Microscopy
Electron
Transmission
Phenols
Spermatocytes
Testis
medicine
Animals
RNA
Messenger
Benzhydryl Compounds
Gene
0105 earth and related environmental sciences
bcl-2-Associated X Protein
Genetics
TUNEL assay
medicine.diagnostic_test
urogenital system
biology.organism_classification
Mitochondria
030104 developmental biology
medicine.anatomical_structure
Endocrine disruptor
Proto-Oncogene Proteins c-bcl-2
Gobiocypris rarus
hormones
hormone substitutes
and hormone antagonists
Water Pollutants
Chemical
Signal Transduction
Zdroj: Aquatic toxicology (Amsterdam, Netherlands). 179
ISSN: 1879-1514
Popis: Bisphenol A (BPA) is an endocrine disruptor, and could induce germ cells apoptosis in the testis of mammals. But whether it could affect fish in the same mechanism has not' been studied till now. In the present study, to investigate the influence of BPA on testis germ cells in fish, adult male rare minnow Gobiocypris rarus were exposed to 225μgL(-1) (0.99μM) BPA for 1, 3 and 9 weeks. Through TdT-mediated dUTP nick end labeling (TUNEL) and transmission electron microscope (TEM) analysis, we found that the amount of apoptotic spermatocytes significantly increased in a time dependent manner following BPA exposure. Western Blot results showed that the ratio of Bcl2/Bax, the important apoptosis regulators in intrinsic mitochondrial apoptotic pathway, was significantly decreased. qPCR showed that mRNA expression of several genes in mitochondrial apoptotic pathway including bcl2, bax, casp9, cytc and mcl1b were significantly changed following BPA exposure. In addition, mRNA expression of meiosis regulation genes (kpna7 and wee2), and genes involved in both apoptosis and meiosis (birc5, ccna1, and gsa1a) were also affected by BPA. Taken together, the present study demonstrated that BPA could induce spermatocytes apoptosis in rare minnow testis, and the apoptosis was probably under regulation of intrinsic mitochondrial apoptotic pathway. Moreover, the spermatocyte apoptosis was likely initiated by BPA induced meiosis arrest.
Databáze: OpenAIRE
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