Pharmaceutical formulation of HSA hybrid coated iron oxide nanoparticles for magnetic drug targeting
| Autor: | Geoffrey Lee, Ralph Heimke-Brinck, Marina Pöttler, Harald Mangge, Jan Zaloga, Frank Dörje, Christoph Alexiou, Rainer Tietze, Eva Baum, Stefan Lyer, Ralf P Friedrich, Gerd Leitinger, Gunter Almer |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Biocompatibility Chemistry Pharmaceutical Pharmaceutical Science Biocompatible Materials Nanotechnology 02 engineering and technology Pharmaceutical formulation Microscopy Atomic Force Ferric Compounds Jurkat Cells Magnetics 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems Adsorption Cell Line Tumor medicine Humans Molecule Serum Albumin General Medicine 021001 nanoscience & nanotechnology Human serum albumin 030104 developmental biology chemistry Targeted drug delivery Chemical engineering Nanoparticles Mitoxantrone 0210 nano-technology Iron oxide nanoparticles Biotechnology Conjugate medicine.drug |
| Zdroj: | European Journal of Pharmaceutics and Biopharmaceutics. 101:152-162 |
| ISSN: | 0939-6411 |
| Popis: | In this work we present a new formulation of superparamagnetic iron oxide nanoparticles (SPIONs) for magnetic drug targeting. The particles were reproducibly synthesized from current good manufacturing practice (cGMP) - grade substances. They were surface coated using fatty acids as anchoring molecules for human serum albumin. We comprehensively characterized the physicochemical core-shell structure of the particles using sophisticated methods. We investigated biocompatibility and cellular uptake of the particles using an established flow cytometric method in combination with microwave-plasma assisted atomic emission spectroscopy (MP-AES). The cytotoxic drug mitoxantrone was adsorbed on the protein shell and we showed that even in complex media it is slowly released with a close to zero order kinetics. We also describe an in vitro proof-of-concept assay in which we clearly showed that local enrichment of this SPION-drug conjugate with a magnet allows site-specific therapeutic effects. |
| Databáze: | OpenAIRE |
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