Nature of Transient Inhibition of Deoxyribonucleic Acid Synthesis in HeLa Cells by Parainfluenza Virus 1 (Sendai)
Autor: | Alexander Kohn, Pinhas Fuchs |
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Rok vydání: | 1971 |
Předmět: |
Formates
Chromatography Paper Ultraviolet Rays Immunology Endogeny Tritium Methylation Microbiology Virus HeLa chemistry.chemical_compound Virology Animal Viruses Humans Nucleotide chemistry.chemical_classification Carbon Isotopes Thymidine monophosphate biology Nucleotides Phosphorus Isotopes DNA biology.organism_classification Molecular biology Sendai virus Parainfluenza Virus 1 Human Radiation Effects chemistry Biochemistry Insect Science Autoradiography Adsorption Thymidine HeLa Cells |
Zdroj: | Journal of Virology. 8:695-700 |
ISSN: | 1098-5514 0022-538X |
DOI: | 10.1128/jvi.8.5.695-700.1971 |
Popis: | Adsorption of ultraviolet-inactivated Sendai virus, at high or low multiplicity, to HeLa cells caused a transient increased incorporation of 3 H-thymidine into the cellular deoxyribonucleic acid (DNA). In HeLa cells synchronized by a double-thymidine block, this increased incorporation of thymidine during the S phase lasted from about 30 to 90 min after virus adsorption. The observations that the kinetics of accumulation of radioactive thymidine in the nucleotide pool did not differ in control and in the virus-treated cells and that the 32 P incorporation into the DNA of the virus-treated cells was inhibited at the same time indicate that the augmented incorporation of 3 H-thymidine into DNA results from a transient block in the endogenous pathway of thymidine synthesis. Chromatographic analysis of the nucleotide pool of the virus-treated cells labeled with 14 C-formate indicates that methylation of deoxyuridine monophosphate to thymidine monophosphate is inhibited. It is suggested that the inhibition is caused by a block of either the thymidilate synthetase or some step in the tetrahydrofolate cycle. |
Databáze: | OpenAIRE |
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