Additional file 3 of Enhanced aluminum tolerance in sugarcane: evaluation of SbMATE overexpression and genome-wide identification of ALMTs in Saccharum spp

Autor: Ribeiro, Ana Paula, Vinecky, Felipe, Duarte, Karoline Estefani, Santiago, Thaís Ribeiro, das Chagas Noqueli Casari, Raphael Augusto, Hell, Aline Forgatti, da Cunha, Bárbara Andrade Dias Brito, Martins, Polyana Kelly, da Cruz Centeno, Danilo, de Oliveira Molinari, Patricia Abrão, de Almeida Cançado, Geraldo Magela, Magalhães, Jurandir Vieira de, Kobayashi, Adilson Kenji, de Souza, Wagner Rodrigo, Molinari, Hugo Bruno Correa
Rok vydání: 2021
DOI: 10.6084/m9.figshare.14880603
Popis: Additional file 3 Supplementary Fig. 3 (a) Schematic representation of the binary vector p7U (DNA Cloning Service, Germany). The vector contains the Sorghum bicolor MATE gene with optimized codon (oSbMATE) under the control of ZmUbi1 promoter. The selective marker is bar (phosphinothricin acetyl transferase) gene under the control of the ZmUbi1 promoter, confers resistance to glufosinate-ammonium herbicide. (b) Agarose gel electrophoresis showing the expected amplicon of SbMATE gene (301 bp) in the transgenic events.
Databáze: OpenAIRE