Impact of epicatechin on fibrin clot structure
| Autor: | Dragan Milenkovic, Thomas Sinegre, Pauline Fully, Christine Morand, Julien Bourdin, Dorian Teissandier, Aurélien Lebreton |
|---|---|
| Přispěvatelé: | Unité de Nutrition Humaine (UNH), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), Service d’Hématologie Biologique [CHU Clermont-Ferrand], CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand-CHU Estaing [Clermont-Ferrand], CHU Clermont-Ferrand, Octapharma |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male Fibrin clot Fibrin Catechin Permeability Turbidity Absorbance 03 medical and health sciences Young Adult 0302 clinical medicine Fibrinolytic Agents Humans Blood Coagulation Epicatechin Pharmacology Coagulation Chromatography Plasma samples biology Chemistry Healthy subjects Fiber size Middle Aged Cardiovascular disease Healthy Volunteers Structure and function Kinetics 030104 developmental biology Permeability (electromagnetism) [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology biology.protein Female 030217 neurology & neurosurgery |
| Zdroj: | European Journal of Pharmacology European Journal of Pharmacology, Elsevier, 2021, 893, pp.173830. ⟨10.1016/j.ejphar.2020.173830⟩ European Journal of Pharmacology, 2021, 893, pp.173830. ⟨10.1016/j.ejphar.2020.173830⟩ |
| ISSN: | 1879-0712 0014-2999 |
| DOI: | 10.1016/j.ejphar.2020.173830⟩ |
| Popis: | International audience; Fibrin clot structure and function are major determinants of thromboembolic diseases. The study aim was to determine the impact of epicatechin (a flavonoid with cardiovascular protective effects) on fibrin clot structure and permeability.Plasma samples from 12 healthy subjects were incubated with increasing concentrations of epicatechin. Turbidity of fibrin clot was analyzed by absorbance measurement at 405 nm. The fibrin clot nanostructure was determined by scanning spectrometry (wavelength from 500 to 800 nm) and fibrin fiber size by electron microscopy. Permeability was analyzed to assess the fibrin clot functional properties.Epicatechin addition increased the maximum absorbance from 0.34 +/- 0.066 (vehicle) to 0.35 +/- 0.077 (P = 0.1), 0.35 +/- 0.072 (P < 0.05) and 0.34 +/- 0.065 (P = 0.5) for 1, 10 and 100 mu M epicatechin, respectively. Epicatechin increased the fibrin clot fiber radius (nm) from 109.2 +/- 3.2 (vehicle) to 108.9 +/- 4.3 (P = 0.9), 110.0 +/- 3.6 (P < 0.05) and 109.5 +/- 3.3 (P = 0.4), and the distance between protofibrils (nm) from 22.2 +/- 1.5 (vehicle) to 22.1 +/- 2.3 (P = 0.9), 22.6 +/- 1.8 (P < 0.05) and 22.3 +/- 1.8 (P = 0.9) for 1, 10 and 100 mu M epicatechin respectively. Electron microscopy confirmed these changes. Fibrin clot permeability, expressed as Darcy's constant (Ks, cm(2)), increased from 2.97 +/- 1.17 (vehicle) to 3.36 +/- 1.21 (P < 0.05), 3.81 +/- 1.41 (P < 0.01) and 3.38 +/- 1.33 (P = 0.9).Upon epicatechin addition, the fibrin clot structure became less dense and more permeable. |
| Databáze: | OpenAIRE |
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