Palmitoleic acid (16:1n7) increases oxygen consumption, fatty acid oxidation and ATP content in white adipocytes
| Autor: | Rui Curi, Andressa Bolsoni Lopes, Maysa Mariana Cruz, Wilson M. T. Kuwabara, Maria Isabel Cardoso Alonso-Vale, Paula B. M. de Andrade, Amanda Rabello Crisma, Roberta Dourado Cavalcante da Cunha de Sá |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism Lipolysis Clinical Biochemistry Adipocytes White 030209 endocrinology & metabolism Oxidative phosphorylation Triglyceride/fatty acid cycle Mitochondrion Bioenergetics Fatty Acids Monounsaturated 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Endocrinology Adenosine Triphosphate Oxygen Consumption 3T3-L1 Cells Adipocytes Palmitoleic acid Citrate synthase Animals lcsh:RC620-627 Beta oxidation Triglycerides chemistry.chemical_classification biology Research Lipogenesis Biochemistry (medical) Fatty Acids Beta-oxidation Fatty acid Lipid Metabolism Mitochondria lcsh:Nutritional diseases. Deficiency diseases 030104 developmental biology chemistry Biochemistry MITOCÔNDRIAS biology.protein Oxidation-Reduction |
| Zdroj: | Repositório Institucional da UNIFESP Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP Lipids in Health and Disease Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Lipids in Health and Disease, Vol 17, Iss 1, Pp 1-12 (2018) |
| Popis: | FAPESP Background: We have recently demonstrated that palmitoleic acid (16:1n7) increases lipolysis, glucose uptake and glucose utilization for energy production in white adipose cells. In the present study, we tested the hypothesis that palmitoleic acid modulates bioenergetic activity in white adipocytes. Methods: For this, 3 T3-L1 pre-adipocytes were differentiated into mature adipocytes in the presence (or absence) of palmitic (16:0) or palmitoleic (16:1n7) acid at 100 or 200 mu M. The following parameters were evaluated:lipolysis, lipogenesis, fatty acid (FA) oxidation, ATP content, oxygen consumption, mitochondrial mass, citrate synthase activity and protein content of mitochondrial oxidative phosphorylation (OXPHOS) complexes. Results: Treatment with 16:1n7 during 9 days raised basal and isoproterenol-stimulated lipolysis, FA incorporation into triacylglycerol (TAG), FA oxidation, oxygen consumption, protein expression of subunits representing OXPHOS complex II, III, and V and intracellular ATP content. These effects were not observed in adipocytes treated with 16:0. Conclusions: Palmitoleic acid, by concerted action on lipolysis, Gamma A esterification, mitochondrial Gamma A oxidation, oxygen consumption and ATP content, does enhance white adipocyte energy expenditure and may act as local hormone. Univ Fed Sao Paulo, Inst Environm Sci Chem & Pharmaceut, Dept Biol Sci, 210 Sao Nicolau St, BR-09913030 Diadema, Brazil Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo, Brazil Cruzeiro do Sul Univ, Inst Phys Act Sci & Sports, Interdisciplinary Postgrad Program Hlth Sci, Sao Paulo, Brazil Univ Fed Espirito Santo, Hlth Sci Ctr, Dept Nursing, Vitoria, Spain Univ Fed Sao Paulo, Inst Environm Sci Chem & Pharmaceut, Dept Biol Sci, 210 Sao Nicolau St, BR-09913030 Diadema, Brazil FAPESP: 2011/51627-8 FAPESP: 2011/51701-3 Web of Science |
| Databáze: | OpenAIRE |
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