IQGAP and mitotic exit network (MEN) proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae
| Autor: | Yulan Xiong, Mark Corbett, Clive Price, Daniel J. Wright, Ewen Munro, James R. Boyne |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Histology Saccharomyces cerevisiae Mitosis Arp2/3 complex Cell Cycle Proteins macromolecular substances Protein Serine-Threonine Kinases medicine.disease_cause Septin Pathology and Forensic Medicine GTP-Binding Proteins medicine Phosphorylation Actin Cytokinesis Monomeric GTP-Binding Proteins Mutation biology Cell Biology General Medicine Actin cytoskeleton biology.organism_classification Actins Cell biology Actin Cytoskeleton Phenotype ras GTPase-Activating Proteins Mitotic exit biology.protein Microtubule-Associated Proteins Protein Kinases |
| Zdroj: | European Journal of Cell Biology. 85:1201-1215 |
| ISSN: | 0171-9335 |
| Popis: | In budding yeast the final stages of the cell division cycle, cytokinesis and cell separation, are distinct events that require to be coupled, both together and with mitotic exit. Here we demonstrate that mutations in genes of the mitotic exit network (MEN) prevent cell separation and are synthetically lethal in combination with both cytokinesis and septation defective mutations. Analysis of the synthetic lethal phenotypes reveals that Iqg1p functions in combination with the MEN components, Tem1p, Cdc15p Dbf20p and Dbf2p to govern the re-polarization of the actin cytoskeleton to either side of the bud neck. In addition phosphorylation of the conserved PCH protein, Hof1p, is dependent upon these activities and requires actin ring assembly. Recruitment of Dbf2p to the bud neck is dependent upon actin ring assembly and correlates with Hof1p phosphorylation. Failure to phosphorylate Hof1p results in the increased stability of the protein and its persistence at the bud neck. These data establish a mechanistic dependency of cell separation upon an intermediate step requiring actomyosin ring assembly. |
| Databáze: | OpenAIRE |
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