Mineralization of 2,4,6-trinitrophenol (picric acid): Characterization and phylogenetic identification of microbial strains
| Autor: | Anna-Louise Reysenbach, Perkins Re, Valli K, Marissa A. Ehringer, Susan M. Barns, Rajan J, Sariaslani Fs, Norman R. Pace, Rehm S |
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| Rok vydání: | 1996 |
| Předmět: |
food.ingredient
Molecular Sequence Data Nitro compound Industrial Waste Bioengineering Picric acid Gram-Positive Bacteria Applied Microbiology and Biotechnology High-performance liquid chromatography Mineralization (biology) chemistry.chemical_compound food Picrates Species Specificity Nitrite Phylogeny chemistry.chemical_classification Chromatography Base Sequence biology Fatty Acids Nocardioides biology.organism_classification Biodegradation Environmental chemistry Biochemistry Energy source Bacteria Biotechnology |
| Zdroj: | Journal of Industrial Microbiology. 16:319-324 |
| ISSN: | 1476-5535 0169-4146 |
| DOI: | 10.1007/bf01570041 |
| Popis: | Four bacterial strains that use picric acid as their sole carbon and energy source were isolated. Mineralization of 14C-UL-picric acid showed that up to 65% of the radioactivity was released as 14CO2. HPLC and UV/Vis spectral analyses indicated complete degradation of picric acid by these organisms. HPLC and LC/MS analyses showed transient formation of 2,4-dinitrophenol during picric acid degradation. Degradation of picric acid was concomitant with stoichiometric release of three moles of nitrite per mole of picric acid. The four picric acid degraders were identified as close relatives of Nocardioides simplex (ATCC 6946) based on their small subunit (16S) rRNA gene sequences. |
| Databáze: | OpenAIRE |
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