Development of a quick and simple detection methodology for foot-and-mouth disease virus serotypes O, A and Asia 1 using a generic RapidAssay Device
| Autor: | Ming Yang, Melissa Goolia, Hilary Bittner, Wanhong Xu, Alfonso Clavijo |
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| Rok vydání: | 2013 |
| Předmět: |
Models
Molecular Veterinary Medicine Serotype Time Factors Protein Conformation medicine.drug_class Molecular Sequence Data Lateral flow immunochromatographic strip test Early detection Antibodies Viral Monoclonal antibody Sensitivity and Specificity Chromatography Affinity Mice Antigen Virology medicine Animals Antigens Viral Mice Inbred BALB C Rapid viral antigen detection biology Clinical Laboratory Techniques Foot-and-mouth disease virus Methodology Antibodies Monoclonal Outbreak Sequence Analysis DNA biology.organism_classification Infectious Diseases Foot-and-Mouth Disease biology.protein Serotype a RNA Viral Female Antibody |
| Zdroj: | Virology Journal |
| ISSN: | 1743-422X |
| DOI: | 10.1186/1743-422x-10-125 |
| Popis: | Background Outbreaks of Foot-and-mouth disease (FMD) have resulted in tremendous economic losses. Thus, the development of a rapid and easily performed test for FMD detection is important for controlling a FMD outbreak and containing its spread. The purpose of this project is to develop a lateral flow immunochromatographic (LFI) strip test for rapid detection of FMD virus serotypes O, A and Asia 1. Methods Specific monoclonal antibodies (mAbs) against each serotype were produced and used as the capture mAbs. A serotype independent mAb was selected and used as the detection mAb with the aim of subsequently developing a multi-serotype strip test. A new generation of the generic RapidAssay Device (gRAD) was used for the test. Result Each strip test can specifically detect the FMDV O, A or Asia 1 viruses, but not other vesicular disease viruses. The LFI strip tests for serotypes A and Asia 1 were able to identify all tested serotype A (n= 39) and Asia 1 field isolates (n=17). Whereas the test for serotype O detected 45 out of 46 field isolates. The sensitivity of this strip test was comparable with the double antibody sandwich ELISA for viral antigen detection. All vesicular fluid and epithelium samples collected from experimentally infected animals with serotype O, A and Asia 1 were identified as positive by the LFI strip test. Swab samples (n=11) collected over the lesion area from experimentally inoculated animals (serotype A) were examined. All of them demonstrated positive results using the LFI serotype A strip test and double antibody sandwich (DAS) ELISA. Conclusions The ability of strip tests to produce rapid results and high specificity makes it a valuable tool for early detection of FMDV O, A and Asia 1 in the field. |
| Databáze: | OpenAIRE |
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