In vitro anti-biofilm activity of bacteriocin from a marine Bacillus sp. strain Sh10 against Proteus mirabilis
| Autor: | Gires Usup, Asmat Ahmad, Fatemeh Shayesteh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Bacterioci Bacteriocin lcsh:QR1-502 Cell morphology 01 natural sciences Microbiology lcsh:Microbiology chemistry.chemical_compound Marine bacteriophage 0502 economics and business Bacillus sp Viability assay Crystal violet 0101 mathematics Proteus mirabilis biology sp Chemistry 010102 general mathematics 05 social sciences Marine bacteria Biofilm biochemical phenomena metabolism and nutrition biology.organism_classification Proteus mirabili bacteria Original Article Anti-biofilm activity 050203 business & management Bacteria |
| Zdroj: | Iranian Journal of Microbiology, Vol 12, Iss 1 (2020) Iranian Journal of Microbiology |
| ISSN: | 2008-4447 2008-3289 |
| Popis: | Background and Objectives: Biofilm formed by Proteus mirabilis strains is one of the most important medical prob- lems especially in the case of device-related urinary tract infections. This study was conducted to evaluate the bacte- riocin produced by a marine isolate of Bacillus sp. Sh10, for it's in vitro inhibitory activity against pre-formed biofilm and in interference with the biofilm-forming of two biofilm-producing bacteria (P. mirabilis UCa4 and P. mirabilis UCe1). Materials and Methods: Sensitivity of two biofilm-producing bacteria (P. mirabilis UCa4 and P. mirabilis UCe1) to bac- teriocin, was investigated in planktonic and biofilm states by cell viability and crystal violet assay, respectively. Scanning electron microscopy (SEM) was also performed to determine the effect of bacteriocin on the morphology of the cells asso- ciated with biofilm. Results: It was found that bacteriocin possessed bactericidal activity to biofilm-forming isolates in the planktonic state. However, bacteriocin interferes with the formation of biofilms and disrupts established biofilms. Bacteriocin reduced biofilm 50 50 formation in the isolates of P. mirabilis UCa4 and P. mirabilis UCe1 with SMIC of 32 and 128 μg/mL, desirable SMIC of bacteriocin for biofilm disruption were 128 and 256 μg/mL, respectively. The SEM results indicated that bacteriocin affected the cell morphology of biofilm-associated cells. Conclusion: The present findings indicated that bacteriocin from Bacillus sp. Sh10 has bactericidal properties against bio- film-forming isolates of P. mirabilis UCa4 and P. mirabilis UCe1 and has the ability to inhibit the formation of biofilm and disrupt established biofilm. |
| Databáze: | OpenAIRE |
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