Ent3p Is a PtdIns(3,5)P2 effector required for protein sorting to the multivesicular body
| Autor: | Sylvie Friant, Delphine Nourrisson, François Letourneur, Eve Isabelle Pécheur, Anne Eugster, Fabrice Michel, Yaya Lefkir |
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| Přispěvatelé: | Friant, Sylvie, Génétique moléculaire, génomique, microbiologie (GMGM), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Deleage, Gilbert |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Time Factors
Epsin Vesicular Transport Proteins Pyridinium Compounds medicine.disease_cause MESH: Multienzyme Complexes MESH: Neuropeptides Mixed Function Oxygenases MESH: Protein Structure Tertiary MESH: Quaternary Ammonium Compounds 0302 clinical medicine Phosphatidylinositol Phosphates Yeasts Protein targeting MESH: Precipitin Tests MESH: Clathrin ENTH domain 0303 health sciences biology MESH: Kinetics Vacuolar lumen Temperature MESH: Mixed Function Oxygenases MESH: Phosphatidylinositol Phosphates Endocytosis Cell biology Protein Transport MESH: Pyridinium Compounds MESH: Endocytosis MESH: Luminescent Proteins MESH: Fungal Proteins MESH: Temp MESH: Protein Sorting Signals MESH: Protein Transport MESH: Mutation Endosome Blotting Western Green Fluorescent Proteins MESH: Sequence Alignment MESH: Carrier Proteins Endosomes [SDV.BC]Life Sciences [q-bio]/Cellular Biology macromolecular substances In Vitro Techniques Protein Sorting Signals MESH: Alkyl and Aryl Transferases MESH: Actins Clathrin General Biochemistry Genetics and Molecular Biology Actin cytoskeleton organization Fungal Proteins 03 medical and health sciences MESH: Green Fluorescent Proteins Multienzyme Complexes medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology MESH: Blotting Western [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Multivesicular Body Molecular Biology [SDV.BC] Life Sciences [q-bio]/Cellular Biology 030304 developmental biology Alkyl and Aryl Transferases Neuropeptides Cell Biology MESH: Adaptor Proteins Vesicular Transport Precipitin Tests Actins Protein Structure Tertiary Quaternary Ammonium Compounds Adaptor Proteins Vesicular Transport Kinetics Luminescent Proteins MESH: Endosomes Mutation Vacuoles biology.protein Carrier Proteins Sequence Alignment 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Cell Developmental Cell, Elsevier, 2003, 5 (3), pp.499-511 Developmental Cell, Elsevier, 2003, 5, pp.499-511 |
| ISSN: | 1534-5807 |
| Popis: | International audience; PtdIns(3,5)P(2) is required for cargo-selective sorting to the vacuolar lumen via the multivesicular body (MVB). Here we show that Ent3p, a yeast epsin N-terminal homology (ENTH) domain-containing protein, is a specific PtdIns(3,5)P(2) effector localized to endosomes. The ENTH domain of Ent3p is essential for its PtdIns(3,5)P(2) binding activity and for its membrane interaction in vitro and in vivo. Ent3p is required for protein sorting into the MVB but not for the internalization step of endocytosis. Ent3p is associated with clathrin and is necessary for normal actin cytoskeleton organization. Our results show that Ent3p is required for protein sorting into intralumenal vesicles of the MVB through PtdIns(3,5)P(2) binding via its ENTH domain.PtdIns(3,5)P(2) is required for cargo-selective sorting to the vacuolar lumen via the multivesicular body (MVB). Here we show that Ent3p, a yeast epsin N-terminal homology (ENTH) domain-containing protein, is a specific PtdIns(3,5)P(2) effector localized to endosomes. The ENTH domain of Ent3p is essential for its PtdIns(3,5)P(2) binding activity and for its membrane interaction in vitro and in vivo. Ent3p is required for protein sorting into the MVB but not for the internalization step of endocytosis. Ent3p is associated with clathrin and is necessary for normal actin cytoskeleton organization. Our results show that Ent3p is required for protein sorting into intralumenal vesicles of the MVB through PtdIns(3,5)P(2) binding via its ENTH domain. |
| Databáze: | OpenAIRE |
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