Identifying abundant immunotherapy and other targets in solid tumors: integrating RNA-seq and Mass Spectrometry proteomics data sets
| Autor: | Wei Zhao, Martin W. McIntosh, Matthew Fitzgibbon, Nigel Clegg, Gordon B. Mills, David A. Crispin, Lindsay Bergan |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Proteomics Cancer Research Sequence analysis medicine.medical_treatment genetic processes RNA-Seq Biology Article Mass Spectrometry 03 medical and health sciences Protein sequencing medicine Humans natural sciences Gene Genetics 030102 biochemistry & molecular biology Sequence Analysis RNA Gene Expression Profiling Cancer Immunotherapy medicine.disease 030104 developmental biology Oncology Cancer cell RNA |
| Popis: | RNA-seq and mass-spectrometry proteomics combined with growing data repositories have greatly increased the capacity to identify candidate proteins or protein sequence variants that share properties of ideal therapy targets, which include being abundant in cancer cells, absent or rare in adult organs (especially vital organs), and shared by many patient tumors. RNA-seq and fixed content arrays can identify genes that are over-expressed or mis-expressed in cancer. RNA-seq is uniquely suited to identifying-cancer specific sequence variants. We review factors relevant for determining whether products of genes that are abundant or differentially abundant in RNA-seq are concordant or discordant with proteins that are identified as abundant or differentially abundant in mass-spectrometry proteomics assays. |
| Databáze: | OpenAIRE |
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