Expression of homologous homeo-box-containing genes in differentiated human teratocarcinoma cells and mouse embryos
| Autor: | Craig A. Hauser, Tammy K. Learned, Alexandra L. Joyner, Robert D. Klein, Gail R. Martin, Robert Tjian |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Embryonal Carcinoma Stem Cells
Transcription Genetic Cellular differentiation Tretinoin Biology General Biochemistry Genetics and Molecular Biology Homology (biology) Cell Line Mouse Teratocarcinoma Mice Sequence Homology Nucleic Acid Gene expression Animals Humans Amino Acid Sequence RNA Messenger Gene Chromosomes Human 16-18 Genetics Nucleic acid sequence Teratoma Chromosome Mapping Nucleic Acid Hybridization Cell Differentiation Embryo Mammalian Molecular biology Chromosome 17 (human) Teratocarcinoma Gene Expression Regulation Genes Neoplastic Stem Cells |
| Zdroj: | Cell. 43(1) |
| ISSN: | 0092-8674 |
| Popis: | Human chromosome 17 contains a cluster of at least three distinct homeo box regions, Hu1, Hu2, and Hu5, within a 20 kb stretch of DNA. A mouse homeo box region, Mu1, which maps to chromosome 11, was isolated and found to contain extensive nucleotide homology with a 4 kb region encompassing the Hu1 homeo box. The conservation of these chromosomal regions between man and mouse was confirmed by nucleotide sequence analysis: approximately 380 bp of DNA are more than 90% homologous and the 61 amino acids of the homeo box domain are perfectly conserved. We found that a human teratocarcinoma cell line expresses high levels of Hu1 homeo-box-containing mRNA only after differentiation of the cells following treatment with retinoic acid. In contrast, mouse teratocarcinoma cells did not express Mu1 homeo-box-containing mRNA at any stage of differentiation, whereas the expression of such transcripts was detected in mouse embryos from 10 to 17 days of gestation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|