Expression of agrin, dystroglycan, and utrophin in normal renal tissue and in experimental glomerulopathies
| Autor: | H.B.P.M. Dijkman, C. J. Ilse Raats, Brenda J.M. Pisa, Karel J.M. Assmann, Birgitte Oppers-Walgreen, Jo H. M. Berden, Jacob van den Born, Marinka A.H. Bakker |
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| Přispěvatelé: | Groningen Kidney Center (GKC), Groningen Institute for Organ Transplantation (GIOT) |
| Rok vydání: | 2000 |
| Předmět: |
RNA
Messenger/genetics Male Immunologische ontstekingsprocessen in de nier Nephrotic Syndrome Utrophin Kidney Glomerulus Wistar Gene Expression Kidney Glomerulonephritis Laminin Inflammatory reactions in the kidneys Dystroglycans Microscopy Immunoelectron Cytoskeletal Proteins/analysis Microscopy Agrin Membrane Proteins/analysis Membrane Glycoproteins Reverse Transcriptase Polymerase Chain Reaction Glomerular basement membrane Kidney/cytology musculoskeletal system Immunohistochemistry Cell biology Glomerular Mesangium Agrin/analysis medicine.anatomical_structure Kidney Glomerulus/cytology Nephrotic Syndrome/chemically induced Betekenis van heparan sulfaat in de GBM voor het ontstaan van proteinurie bij experimentele glomerulopathiÙen musculoskeletal diseases medicine.medical_specialty congenital hereditary and neonatal diseases and abnormalities Membrane Glycoproteins/analysis animal structures Kidney Cortex Immunoelectron microscopy Podocyte foot Biology Pathology and Forensic Medicine Significance of heparan sulfate in the GBM for proteinuria in experimental glomerular diseases Glomerular Mesangium/cytology Internal medicine medicine Dystroglycan Animals RNA Messenger Rats Wistar Immunoelectron urogenital system Doxorubicin/administration & dosage Kidney Cortex/chemistry Membrane Proteins Rats Glomerulonephritis/genetics Cytoskeletal Proteins Endocrinology Doxorubicin biology.protein RNA Messenger/genetics Pikachurin Regular Articles |
| Zdroj: | American Journal of Pathology, 156, pp. 1749-1765 American Journal of Pathology, 156, 1749-1765 The American Journal of Pathology, 156(5), 1749-1765. ELSEVIER SCIENCE INC |
| ISSN: | 0002-9440 |
| Popis: | The dystrophin-glycoprotein complex, which comprises alpha- and beta-dystroglycan, sarcoglycans, and utrophin/dystrophin, links the cytoskeleton to agrin and laminin in the basal lamina in muscle and epithelial cells. Recently, agrin was identified as a major heparan sulfate proteoglycan in the glomerular basement membrane. In the present study, we found mRNA expression for agrin, dystroglycan, and utrophin in kidney cortex, isolated glomeruli, and cultured podocytes and mesangial cells. In immunofluorescence, agrin was found in the glomerular basement membrane. The antibodies against alpha- and beta-dystroglycan and utrophin revealed a granular podocyte-like staining pattern along the glomerular capillary wall. With immunoelectron microscopy, agrin was found in the glomerular basement membrane, dystroglycan was diffusely found over the entire cell surface of the podocytes, and utrophin was localized in the cytoplasm of the podocyte foot processes. In adriamycin nephropathy, a decrease in the glomerular capillary wall staining for dystroglycan was observed probably secondary to the extensive fusion of foot processes. Immunoelectron microscopy showed a different distribution pattern as compared to the normal kidney, with segmentally enhanced expression of dystroglycan at the basal side of the extensively fused podocyte foot processes. In passive Heymann nephritis we observed no changes in the staining intensity and distribution of the dystrophin-glycoprotein complex by immunofluorescence and immunoelectron microscopy. From these data, we conclude that agrin, dystroglycan, and utrophin are present in the glomerular capillary wall and their ultrastructural localization supports the concept that these molecules are involved in linking the podocyte cytoskeleton to the glomerular basement membrane. |
| Databáze: | OpenAIRE |
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