Maturation-dependent changes of angiotensin receptor expression in fowl
| Autor: | Karin Ruijtenbeek, Pierre Corvol, H. A. J. Struijker Boudier, J. G. R. De Mey, Yimu Yang, Hiroko Nishimura, C. Hubert, J.-M. Gasc |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Angiotensin receptor Physiology Renal glomerulus Fowl Kidney Glomerulus Chick Embryo Receptor Angiotensin Type 1 Renin-Angiotensin System Physiology (medical) Internal medicine Adrenal Glands Gene expression Renin–angiotensin system medicine Animals Vasoconstrictor Agents Aorta Abdominal RNA Messenger Receptor In Situ Hybridization Receptors Angiotensin Angiotensin II receptor type 1 biology Reverse Transcriptase Polymerase Chain Reaction Chemistry Angiotensin II Gene Expression Regulation Developmental biology.organism_classification Vasodilation Endocrinology cardiovascular system Chickens Signal Transduction |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 285:R231-R242 |
| ISSN: | 1522-1490 0363-6119 |
| Popis: | An angiotensin (ANG) receptor homologous to the type 1 receptor (AT1) has been cloned in chickens (cAT1). We investigated whether cAT1expression in various tissues shows maturation/age-dependent changes. cAT1mRNA levels detected in renal glomeruli [in situ hybridization (ISH)] and kidney extract (RT-PCR) are significantly ( P < 0.01) higher in 19-day embryos (EB) than in chicks (CH, 2–3 wk) and pullets/cockerels (PL/CK, 14–16 wk). The levels in adrenal glands (concentrated in subcapsular regions) are high in EB and further increased in CH and PL/CK. cAT1mRNA is also detectable in smooth muscle (SM)/adventitia of EB and CH aorta and in the adventitia, but not SM, from PL/CK aortas. The endothelia from small arteries and arterioles, but not from aorta, express cAT1mRNA (ISH). In all age groups, ANG II induces profound endothelium-dependent relaxation of abdominal aorta, partly (37–47%) inhibitable ( P < 0.01) by Nω-nitro-l-arginine methyl ester (l-NAME, 10-4M), suggesting the presence of ANG receptor in endothelium. l-NAME-resistant ANG II relaxation, examined in a limited number of EB or CH aortas, was reduced by 125 mM K+or apamin plus charybdotoxin. The results suggest that 1) cAT1is present in kidney, adrenal gland, and vascular endothelium (heterogeneity exists among arteries) of EB, CH, and PL/CK, and in aortic SM/adventitia of EB/CH but only in adventitia of PL/CK; 2) levels of cAT1gene expression change during maturation in a tissue-specific manner; and 3) ANG II-induced relaxation may be partly attributable to nitric oxide and potassium channel activation. |
| Databáze: | OpenAIRE |
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