Dual promoter lentiviral vector generates transgenic mice expressing E2-CSFV glycoprotein in their milk, but impairs early identification of transgenic embryos
| Autor: | Yanet Prieto Carratalá, Lester Suárez Amarán, Jorge Roberto Toledo Alonso, Parra Pereira Natalie Carolina, Silvana P. Jimenez Chaves, Oliberto Sánchez Ramos, Silvia Gómez Puerta |
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| Rok vydání: | 2010 |
| Předmět: |
Genetically modified mouse
Time Factors Transgene Genetic Vectors Mice Transgenic Cell Separation Biology law.invention Viral vector Green fluorescent protein Mice Food Animals Viral Envelope Proteins law Animals Small Animals Promoter Regions Genetic Microinjection Gene Cells Cultured Equine Lentivirus Gene Transfer Techniques Embryo Mammalian Molecular biology Mice Inbred C57BL Milk Recombinant DNA Animal Science and Zoology Female Expression cassette |
| Zdroj: | Theriogenology. 75(7) |
| ISSN: | 1879-3231 |
| Popis: | Lentiviral vectors containing the green fluorescent protein gene have been successfully used to select transgenic embryos before transfer to a surrogate mother. However, there are apparently no reports regarding early detection of transgenic embryos using a lentiviral vector carrying an additional transcription unit for tissue-specific expression of a valuable protein. In this study, two HIV-based lentiviral vectors were constructed. The first one contained the green fluorescent protein (GFP) coding sequence driven by the early SV40 promoter (Lv-G), whereas the other contained an additional transcription unit for the expression of E2 glycoprotein from classical swine fever virus, driven by a 1.5 kb αS1casein promoter from water buffalo (Lv-αS1cE2hisG). Microinjection of single-cell mouse embryos with Lv-G lentiviral vector rendered embryos which were GFP-positive, beginning at the four-cell stage. Of 33 mice born, 28 (81%) carried the transgene DNA and 15 (55.5%) were GFP-positive. Microinjection of Lv-αS1cE2hisG lentiviral vector yielded 28 mice born; although 24 (85%) carried the transgene DNA, none were GFP-positive, suggesting that the tissue-specific expression cassette interfered with expression of the ubiquitous trancriptional unit. In Lv-αS1cE2hisG transgenic mice, E2his was expressed in milk as a homodimer (at concentrations ≤ 0.422 mg/mL). This was apparently the first report of expression of a recombinant protein in the milk of transgenic animals generated by lentiviral transgenesis. |
| Databáze: | OpenAIRE |
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