Kutzneria buriramensis sp. nov., isolated from soil, and emended description of the genus Kutzneria
| Autor: | Jean-Jacques Sanglier, Suwanee Chunhametha, Wipaporn Ngaemthao, Tomohiko Tamura, Chanwit Suriyachadkun |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Rhamnose Molecular Sequence Data Peptidoglycan Microbiology chemistry.chemical_compound Phylogenetics RNA Ribosomal 16S Actinomycetales Botany Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics Mycelium Base Composition biology Strain (chemistry) Fatty Acids Vitamin K 2 Sequence Analysis DNA General Medicine Thailand biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques chemistry Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 63:47-52 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.036533-0 |
| Popis: | A Gram-staining-positive, filamentous bacterium, which developed large globose sporangia at the ends of long sporangiophores on aerial mycelium, was isolated from dry soil collected in a deciduous forest in Thailand. The cell-wall peptidoglycan of the novel bacterium, which was designated strain A-T 1846T, contained meso-diaminopimelic acid and the whole-cell sugars comprised rhamnose, ribose, mannose, glucose and galactose. The predominant menaquinone was MK-9(H4). Mycolic acids were not detected. The identified phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol. The predominant cellular fatty acids were iso-C16 : 0, iso-C16 : 0 2-OH and C16 : 0 10-methyl. The G+C content of the genomic DNA was 72 mol%. The phenotypic and chemotaxonomic characteristics of the novel strain resembled those of established members of the genus Kutzneria . Phylogenetic analyses based on 16S rRNA gene sequences also indicated that this strain belonged to the genus Kutzneria but as a representative of a putative novel species. In hybridization experiments, the DNA–DNA relatedness values recorded between the novel strain and established members of the genus Kutzneria all fell well below 70 %. Based on the phenotypic, chemotaxonomic and genotypic evidence and the results of the DNA–DNA hybridizations, strain A-T 1846T represents a novel species in the genus Kutzneria , for which the name Kutzneria buriramensis sp. nov. is proposed. The type strain is A-T 1846T ( = BCC 29373T = NBRC 107931T). An emended description of the genus Kutzneria is provided. |
| Databáze: | OpenAIRE |
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