Simultaneous determination of curcumin, tetrahydrocurcumin, quercetin, and paeoniflorin by UHPLC-MS/MS in rat plasma and its application to a pharmacokinetic study
| Autor: | Dake Cai, Weilan Yu, Dingsheng Wen, Yanping Guan, Haining Gan, Guoping Zhong, Biaochang Lao, Xiaolin Liu, Weibang Yu, Fulin Jiang, Juntao Zheng |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Electrospray Curcumin Formic acid Metabolite Liquid-Liquid Extraction Clinical Biochemistry Ethyl acetate Administration Oral Pharmaceutical Science Mass spectrometry 01 natural sciences Analytical Chemistry Rats Sprague-Dawley chemistry.chemical_compound Glucosides Pharmacokinetics Limit of Detection Tandem Mass Spectrometry Drug Discovery Animals Drug Interactions Chromatography High Pressure Liquid Spectroscopy Chromatography 010405 organic chemistry Chemistry 010401 analytical chemistry Paeoniflorin Rats 0104 chemical sciences Monoterpenes Drug Therapy Combination Quercetin Drug Monitoring Drugs Chinese Herbal |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 172:58-66 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2019.04.033 |
| Popis: | Curcumin (CUR) is a bioactive compound present in many composite prescriptions of traditional Chinese medicine together with quercetin (QR) and paeoniflorin (PF). Little is known about the influence of QR and PF on the absorption and metabolism of CUR when the three compounds are orally co-administered. In this study, a rapid, sensitive, and reliable ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated for the simultaneous determination of CUR, tetrahydrocurcumin (THC), QR, and PF in rat plasma by using tinidazole as the internal standard (IS). A liquid-liquid extraction method with ethyl acetate was used to pre-treat the plasma samples. Chromatographic separation was conducted on a C18 column with isocratic elution using acetonitrile and 0.1% formic acid water solution (80:20, v/v) as the mobile phase at the flow rate of 0.3 mL/min. A TSQ Quantum Access Max API mass spectrometer equipped with electrospray ionisation (ESI) source in selection reaction monitoring (SRM) mode was employed to determine transitions of m/z 369.0 → 176.9, 373.1 → 137.0, 303.0 → 228.9, 478.9 → 120.9, 248.1 → 121.0 for CUR, THC, QR, PF, and IS, respectively. The selectivity, precision, accuracy, extraction recovery, matrix effect, and stability of the method were validated. This developed and validated method was successfully applied in the pharmacokinetic study of CUR, THC, QR, and PF in rats. The effects of QR and PF on the pharmacokinetics of CUR and its metabolite, THC, were evaluated in the plasma of Sprague-Dawley rats that were orally co-administered CUR, QR, and PF. The results showed that the combined use of QR, PF, and CUR has a possible influence on the metabolism and excretion of CUR. Our work provides a fundamental method for the rapid simultaneous determination of CUR, THC, QR, and PF in rat plasma. Furthermore, this study will provide a basic method for the analysis of pharmacokinetic interaction of CUR, QR, and PF and offer a scientific basis for a possible combination therapy with the three compounds. |
| Databáze: | OpenAIRE |
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