A mimetic peptide of α2,6-sialyllactose promotes neuritogenesis
| Autor: | Weijiang Zhao, Hui Fan Shen, Melitta Schachner, Yong Jian Mi, Shuang Xi Chen, Jia Hui He |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Phage display Neurite Central nervous system Peptide medicine.disease_cause central nervous system cerebellar granule neurons mimetic peptide neural cell adhesion molecule l1 neuritogenesis neurodegenerative disease neuronal survival oxidative stress phage display sambucus nigra lectin α2 lcsh:RC346-429 Pathogenesis 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience medicine lcsh:Neurology. Diseases of the nervous system chemistry.chemical_classification biology Chemistry Sambucus nigra lectin Lectin α2 6-sialyllactose neural cell adhesion molecule L1 Amino acid Cell biology 030104 developmental biology medicine.anatomical_structure biology.protein 6-sialyllactose 030217 neurology & neurosurgery Oxidative stress Research Article |
| Zdroj: | Neural Regeneration Research Neural Regeneration Research, Vol 15, Iss 6, Pp 1058-1065 (2020) |
| ISSN: | 1673-5374 |
| DOI: | 10.4103/1673-5374.270313 |
| Popis: | Oxidative stress contributes to the pathogenesis of neurodegenerative diseases. With the aim to find reagents that reduce oxidative stress, a phage display library was screened for peptides mimicking α2,6-sialyllactose (6′-SL), which is known to beneficially influence neural functions. Using Sambucus nigra lectin, which specifically binds to 6′-SL, we screened a phage display library and found a peptide comprising identical sequences of 12 amino acids. Mimetic peptide, reverse peptide and scrambled peptide were tested for inhibition of 6′-SL binding to the lectin. Indeed, lectin binding to 6′-SL was inhibited by the most frequently identified mimetic peptide, but not by the reverse or scrambled peptides, showing that this peptide mimics 6′-SL. Functionally, mimetic peptide, but not the reverse or scrambled peptides, increased viability and expression of neural cell adhesion molecule L1 in SK-N-SH human neuroblastoma cells, and promoted survival and neurite outgrowth of cultured mouse cerebellar granule neurons challenged by H2O2-induced oxidative stress. The combined results indicate that the 6′-SL mimetic peptide promotes neuronal survival and neuritogenesis, thus raising hopes for the treatment of neurodegenerative diseases. This study was approved by the Medical Ethics Committee of Shantou University Medical College, China (approval No. SUMC 2014-004) on February 20, 2014. |
| Databáze: | OpenAIRE |
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